Håskjold E, Refsum S B, Bjerknes R
Institute of Pathology, University of Oslo, Rikshospitalet, Norway.
APMIS. 1990 Nov;98(11):969-76. doi: 10.1111/j.1699-0463.1990.tb05022.x.
A stathmokinetic method (using Colcemid) and the [3H]thymidine technique (pulse labelling with tritiated thymidine, [3H]TdR) have been evaluated in the rat corneal epithelium. The dose is not of critical importance for the Colcemid method, thus indicating an all or nothing effect within the dose range studied. A one point estimate is sufficient to calculate the mitotic rate (MR), and in the rat corneal epithelium a 4 h accumulation period is recommended. After administration of [3H]TdR there is an increasing response with increasing dose, followed by a levelling off at higher doses. It seems reasonable to use the lowest maximal effective dose. The labelling index (LI) can be reliably registered 1 h after administration of the drug. For each of the drugs we found corresponding results after topical application and intraperitoneal injection. Hence, topical application of small doses of both Colcemid and [3H]TdR makes interesting in vivo experiments on larger animals and even on human beings possible. Due to the extreme regularity of the corneal epithelium this part of the eye is an interesting organ for cell kinetic studies and provides an excellent tool for evaluating cell kinetic methods.
一种静止期动力学方法（使用秋水仙酰胺）和[3H]胸腺嘧啶核苷技术（用氚标记的胸腺嘧啶核苷[3H]TdR进行脉冲标记）已在大鼠角膜上皮中进行了评估。对于秋水仙酰胺方法，剂量并非至关重要，这表明在所研究的剂量范围内存在全或无效应。单点估计足以计算有丝分裂率（MR），在大鼠角膜上皮中，建议采用4小时的累积期。给予[3H]TdR后，随着剂量增加反应增强，随后在更高剂量时趋于平稳。使用最低的最大有效剂量似乎是合理的。给药1小时后可可靠记录标记指数（LI）。对于每种药物，我们在局部应用和腹腔注射后都得到了相应的结果。因此，局部应用小剂量的秋水仙酰胺和[3H]TdR使得对大型动物甚至人类进行有趣的体内实验成为可能。由于角膜上皮的极端规律性，眼睛的这一部分是细胞动力学研究的一个有趣器官，并为评估细胞动力学方法提供了一个极好的工具。
