Emerson J B, Keady P B, Clements N, Morgan E E, Awerbuch J, Miller S L, Fierer N
Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, CO, USA.
Department of Mechanical Engineering, University of Colorado at Boulder, Boulder, CO, USA.
Indoor Air. 2017 May;27(3):576-586. doi: 10.1111/ina.12347. Epub 2016 Oct 31.
Our homes are microbial habitats, and although the amounts and types of bacteria in indoor air have been shown to vary substantially across residences, temporal variability within homes has rarely been characterized. Here, we sought to quantify the temporal variability in the amounts and types of airborne bacteria in homes, and what factors drive this variability. We collected filter samples of indoor and outdoor air in 15 homes over 1 year (approximately eight time points per home, two per season), and we used culture-independent DNA sequencing approaches to characterize bacterial community composition. Significant differences in indoor air community composition were observed both between homes and within each home over time. Indoor and outdoor air community compositions were not significantly correlated, suggesting that indoor and outdoor air communities are decoupled. Indoor air communities from the same home were often just as different at adjacent time points as they were across larger temporal distances, and temporal variation correlated with changes in environmental conditions, including temperature and relative humidity. Although all homes had highly variable indoor air communities, homes with the most temporally variable communities had more stable, lower average microbial loads than homes with less variable communities.
我们的家是微生物栖息地，尽管室内空气中细菌的数量和种类已被证明在不同住宅间有很大差异，但家庭内部的时间变异性却很少被描述。在这里，我们试图量化家庭中空气传播细菌数量和种类的时间变异性，以及驱动这种变异性的因素。我们在1年时间里收集了15个家庭的室内和室外空气过滤样本（每个家庭约8个时间点，每个季节2个），并使用非培养DNA测序方法来表征细菌群落组成。随着时间的推移，在不同家庭之间以及每个家庭内部都观察到了室内空气群落组成的显著差异。室内和室外空气群落组成没有显著相关性，这表明室内和室外空气群落是解耦的。来自同一家庭的室内空气群落在相邻时间点的差异往往与在较大时间跨度上的差异一样大，并且时间变化与环境条件的变化相关，包括温度和相对湿度。尽管所有家庭的室内空气群落都高度可变，但群落时间变异性最大的家庭比变异性较小的家庭具有更稳定、更低的平均微生物负荷。
