乌贼在盲肠结扎和穿刺诱导的脓毒症动物模型中的治疗潜力效应探索。

Exploration of the therapeutic potential effect of Sepia officinalis in animal model of sepsis induced by cecal ligation and puncture.

作者信息

Abd El-Latif Asmaa Abd El-Aleem, Sayed Amany Ahmed, Soliman Amel Mahmoud, Fahmy Sohair Ramadan

机构信息

Zoology Department, Faculty of Science, Cairo University, 12613, Giza, Egypt.

出版信息

Injury. 2016 Dec;47(12):2709-2717. doi: 10.1016/j.injury.2016.10.003. Epub 2016 Oct 6.

DOI:10.1016/j.injury.2016.10.003

PMID:27743598

Abstract

OBJECTIVE

The present investigation explored the therapeutic potential effect of Sepia officinalis body tissue (SOBT) and Sepia officinalis polysaccharide (SOP) extracts, in animal model of sepsis [induced by cecal ligation and puncture (CLP)].

MATERIALS AND METHODS

Experimental animals were divided into 4 groups, Group 1: Sham control rats. Group 2: Septic rats. Group 3: Septic rats treated with methanolic extract of Sepia officinalis body tissue (SOBT) (500mg/kg body weight) for 2days. Group 4: Septic rats treated with Sepia officinalis polysaccharide (SOP) extract (200mg/kg body weight) for 2days.

RESULTS

The antioxidant activity of SOBT and SOP was proven by DPPH test. CLP-induced liver and kidney toxicities showed by an increase in the ALAT, ASAT, γGT, ALP, creatinine, BUN and uric acid concentrations in serum. Moreover, CLP-induced oxidative stress in liver and kidney evidenced by the increase of MDA levels, decrease in GSH concentrations and decrease in the enzymatic antioxidants (SOD, CAT, GST). In addition, CLP caused decrease in CYP1A2 content in liver.

CONCLUSIONS

Our findings demonstrate the therapeutic efficacy of SOBT and SOP in liver and kidney disorders. Therefore this study suggests that SOBT and SOP could be a potential therapeutic agents for sepsis treatment.

摘要

目的

本研究探讨乌贼身体组织提取物（SOBT）和乌贼多糖（SOP）在盲肠结扎和穿刺（CLP）诱导的脓毒症动物模型中的治疗潜在效果。

材料与方法

将实验动物分为4组，第1组：假手术对照大鼠。第2组：脓毒症大鼠。第3组：用乌贼身体组织甲醇提取物（SOBT）（500mg/kg体重）治疗2天的脓毒症大鼠。第4组：用乌贼多糖（SOP）提取物（200mg/kg体重）治疗2天的脓毒症大鼠。

结果

通过DPPH试验证明了SOBT和SOP的抗氧化活性。CLP诱导的肝肾毒性表现为血清中丙氨酸转氨酶（ALAT）、天冬氨酸转氨酶（ASAT）、γ-谷氨酰转移酶（γGT）、碱性磷酸酶（ALP）、肌酐、尿素氮（BUN）和尿酸浓度升高。此外，CLP诱导的肝肾氧化应激表现为丙二醛（MDA）水平升高、谷胱甘肽（GSH）浓度降低以及酶促抗氧化剂（超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽S-转移酶（GST））减少。另外，CLP导致肝脏中细胞色素P450 1A2（CYP1A2）含量降低。