Eco-auditing Group, CSIR-National Botanical Research Institute, Lucknow 226001, India; Department of Biochemistry, Banaras Hindu University, Varanasi 221001, India.
Eco-auditing Group, CSIR-National Botanical Research Institute, Lucknow 226001, India.
J Environ Sci (China). 2016 Oct;48:112-119. doi: 10.1016/j.jes.2015.12.034. Epub 2016 Apr 2.
The arsenic tolerant bacterial strains Staphylococcus arlettae (NBRIEAG-6), Staphylococcus sp. (NBRIEAG-8) and Brevibacillus sp. (NBRIEAG-9) were tested for their roles in enhancing plant growth and induction of stress-related enzymes in rice (Oryza sativa L. cv. NDR-359) plants at two different concentrations, 30 and 15mg/kg of As(V) and As(III), respectively. An experiment was conducted to test the effect of these strains on plant growth promotion and arsenic uptake. We found 30%-40% reduction in total As uptake in bacteria-inoculated plants, with increased plant growth parameters compared to non-inoculated plants. Moreover, the bacteria-inoculated plants showed reduced activity of total glutathione (GSH) and glutathione reductase (GR) compared to their respective controls, which suggests the bacteria-mediated reduction of oxidative stress in plants. Thus, these strains were found to be beneficial in terms of the biochemical and physiological status of the plants under arsenic stress conditions. Furthermore, one-way ANOVA and principal component analysis (PCA) on enzymatic and non-enzymatic assays also revealed clear variations. The results support the distinction between control and treatments in both shoots and roots. Therefore, this study demonstrates the potential of rhizobacteria in alleviating arsenic stress in rice plants.
耐砷细菌菌株葡萄球菌（NBRIEAG-6）、葡萄球菌（NBRIEAG-8）和短芽孢杆菌（NBRIEAG-9）分别在 30 和 15mg/kg 的 As(V)和 As(III)两种不同浓度下，测试了其在水稻（Oryza sativa L. cv. NDR-359）植株中促进植物生长和诱导与胁迫相关酶的作用。进行了一项实验来测试这些菌株对植物生长促进和砷吸收的影响。我们发现，与未接种的植物相比，接种细菌的植物中总砷吸收减少了 30%-40%，并且植物生长参数增加。此外，与各自的对照相比，接种细菌的植物中总谷胱甘肽（GSH）和谷胱甘肽还原酶（GR）的活性降低，这表明细菌介导的植物氧化应激减轻。因此，这些菌株在砷胁迫条件下对植物的生化和生理状态有益。此外，酶和非酶测定的单向方差分析（ANOVA）和主成分分析（PCA）也揭示了明显的差异。结果支持了在茎和根中对照和处理之间的区分。因此，本研究证明了根际细菌在缓解水稻植株砷胁迫方面的潜力。
