Silva Chenia Frutuoso, Felzemburgh Victor de Araújo, Moreno Amanda Dourado, Meneses José Valber Lima, Barbosa Júnior Aryon de Almeida, Barreto Isabela Cerqueira, Miguel Fúlvio Borges
Universidade Federal da Bahia - Institute of Health Sciences - Laboratory of Tissue Bioengineering and Biomaterials - Salvador (BA) - Brazil.
Universidade Federal da Bahia - Post-graduation Program in Interactive Processes of the Organs and Systems - Salvador (BA) - Brazil.
Acta Cir Bras. 2024 Sep 30;39:e396724. doi: 10.1590/acb396724. eCollection 2024.
To describe an experimental surgical model in rats using a dual-plane technique for evaluation of biomaterials in an in-vivo silicone implant coverage.
This study was developed following the ISO 10993-6 standard. In this study, 40 male Wistar rats weighing between 250 and 350 g were used, distributed into two groups: experimental, biomaterial superimposed on the minimammary prosthesis (MP); and control, MP without implantation of the biomaterial, with eight animals at each biological point: 1, 2, 4, 12, and 26 weeks. Thus, at the end of biological points (1, 2, 4, 12, and 26 weeks; n = 8 animals per week), the tissue specimens achieved were fixed in buffered formalin and stained with hematoxylin-eosin.
Macroscopically, throughout the study, no postoperative complications were apparent. In the histological analysis, it was possible to observe the evolution of the inflammatory response, tissue repair, and fibrous capsule during the biological points.
The experimental model described in this study proved to be suitable for evaluating the biomaterial used in the coverage of breast silicone implants.
描述一种在大鼠中使用双平面技术的实验性手术模型，用于评估生物材料在体内对硅胶植入物的覆盖情况。
本研究按照ISO 10993 - 6标准开展。在本研究中，使用了40只体重在250至350克之间的雄性Wistar大鼠，分为两组：实验组，将生物材料叠加在最小乳腺假体（MP）上；对照组，MP未植入生物材料，在每个生物学时间点（1、2、4、12和26周）各有8只动物。因此，在生物学时间点结束时（1、2、4、12和26周；每周n = 8只动物），获取的组织标本用缓冲福尔马林固定，并用苏木精 - 伊红染色。
宏观上，在整个研究过程中，未观察到明显的术后并发症。在组织学分析中，可以观察到在各个生物学时间点炎症反应、组织修复和纤维囊的演变情况。
本研究中描述的实验模型被证明适用于评估用于覆盖乳房硅胶植入物的生物材料。
