Ostmeier H, Suter L
Fachklinik Hornheide, Münster, Federal Republic of Germany.
Arch Dermatol Res. 1989;281(3):173-7. doi: 10.1007/BF00456388.
We investigated whether two parameters of proliferative activity - mitotic rate and Ki-67 positive cells - are interchangeable. The mitotic rate was assessed on paraffin-embedded sections, Ki-67 positive cells were immunohistologically determined in frozen tissue. A poor correlation (correlation coefficient r = 0.57) was found between both parameters. The proliferative activity was often not homogeneously distributed in the tested tumors. However, this is a major reason for the observed difference only in thin melanomas (less than 1.5 mm) as seen by comparison of tumors with homogeneous and inhomogeneous proliferative activity. We assume that arrest of cells in different stages of the cell cycle - variable from melanoma to melanoma - is the major reason for the observed discrepancy between mitotic rate and Ki-67 positive cells in tumors of 1.5 mm and thicker. The mean number of Ki-67 positive cells increased with tumor thickness. The stability of the Ki-67 antigen towards freezing, thawing, and formalin was studied.
我们研究了增殖活性的两个参数——有丝分裂率和Ki-67阳性细胞——是否可相互替代。有丝分裂率在石蜡包埋切片上评估，Ki-67阳性细胞在冰冻组织中通过免疫组织化学方法测定。发现这两个参数之间相关性较差（相关系数r = 0.57）。在受试肿瘤中，增殖活性通常分布不均一。然而，这仅是在薄黑色素瘤（小于1.5毫米）中观察到差异的主要原因，通过比较增殖活性均一和不均一的肿瘤可见。我们推测，细胞在细胞周期不同阶段的停滞——因黑色素瘤而异——是在1.5毫米及更厚的肿瘤中观察到的有丝分裂率与Ki-67阳性细胞之间差异的主要原因。Ki-67阳性细胞的平均数量随肿瘤厚度增加。研究了Ki-67抗原对冷冻、解冻和福尔马林的稳定性。
