Pierard G E, Pierard-Franchimont C
Am J Dermatopathol. 1984 Summer;6 Suppl:317-23.
The proliferative rates of cells of malignant melanomas were studied by autoradiography after incorporation of tritiated thymidine in them. There is a relationship between the proportion of melanomatous cells synthesizing DNA and the maximum thickness of the neoplasm. Our data suggest that there are two biologically distinct steps in the evolution of malignant melanoma that are closely related to the direction of growth of the neoplasm (horizontal or vertical) but unrelated to the clinicopathological pattern (superficial spreading, nodular or lentiginous). Malignant melanomas with high risk for early metastases are characterized by higher labeling indices for tritiated thymidine (greater than 1.5%) and by greater numbers of melanomatous cells synthesizing DNA (greater than 40/mm2 at the lateral margins of the neoplasms).
通过在恶性黑色素瘤细胞中掺入氚标记的胸腺嘧啶核苷后进行放射自显影，研究了恶性黑色素瘤细胞的增殖率。合成DNA的黑色素瘤细胞比例与肿瘤的最大厚度之间存在关联。我们的数据表明，恶性黑色素瘤的演变存在两个生物学上不同的阶段，这两个阶段与肿瘤的生长方向（水平或垂直）密切相关，但与临床病理模式（浅表扩散型、结节型或雀斑型）无关。具有早期转移高风险的恶性黑色素瘤的特征是氚标记胸腺嘧啶核苷的标记指数较高（大于1.5%），且合成DNA的黑色素瘤细胞数量较多（在肿瘤侧缘大于40/mm²）。
