Cui Xiangrong, Jing Xuan, Wu Xueqing, Yan Meiqin
Reproductive Medicine Center, Children's Hospital of Shanxi and Women Health Center of Shanxi, Taiyuan, Shanxi 030000, P.R. China.
Clinical Laboratory, Shanxi Province People's Hospital, Taiyuan, Shanxi 030000, P.R. China.
Mol Med Rep. 2016 Nov;14(5):4659-4665. doi: 10.3892/mmr.2016.5840. Epub 2016 Oct 12.
Male infertility is a complex, multifactorial and polygenic disease that contributes to ~50% cases of infertility. Previous studies have demonstrated that excess weight and obesity factors serve an important role in the development of male infertility. An increasing number of studies have reported that resveratrol may regulate the response of cells to specific stimuli that induce cell injury, as well as decrease germ cell apoptosis in mice or rats. In the present study, the semen quality and serum sex hormone levels were evaluated in 324 men, which included 73 underweight, 82 normal weight, 95 overweight and 74 obese men. All patients were referred to The Reproductive Medicine Center of Shanxi Women and Infants Hospital (Taiyuan, China) between January 2013 and January 2015. The aim of the present study was to investigate the effects of resveratrol treatment on the motility, plasma zinc concentration and acrosin activity of sperm from obese males. The sperm concentration, normal sperm morphology, semen volumes, DNA fragmentation rates and testosterone levels in men from the overweight and obese groups were markedly decreased when compared with men in the normal weight group. In addition, the progressive motility, seminal plasma zinc concentration and spermatozoa acrosin activity were notably decreased in the obese group compared with the normal weight group. However, estradiol levels were significantly increased in the overweight, obese and underweight groups compared with the normal weight group. Notably, semen samples from obese males with astenospermia treated with 0‑100 µmol/l resveratrol for 30 min demonstrated varying degrees of improvement in sperm motility. When these semen samples were treated with 30 µmol/l resveratrol, sperm motility improved when compared to other doses of resveratrol. Therefore, 30 µmol/l resveratrol was selected for further experiments. Upon treatment of semen samples with resveratrol (30 µmol/l) for 30 min, the seminal plasma zinc concentration and spermatozoa acrosin activity increased significantly in the experimental group compared with the control group. These data suggest that male obesity negatively impacts on male reproductive potential, not only through altering hormone levels, but also by directly altering sperm function. In addition, resveratrol may have a therapeutic and protective effect against obesity-induced abnormalities in semen.
男性不育是一种复杂的、多因素和多基因疾病，约占不育病例的50%。先前的研究表明，超重和肥胖因素在男性不育的发生发展中起重要作用。越来越多的研究报道，白藜芦醇可能调节细胞对诱导细胞损伤的特定刺激的反应，并减少小鼠或大鼠的生殖细胞凋亡。在本研究中，对324名男性的精液质量和血清性激素水平进行了评估，其中包括73名体重过轻、82名体重正常、95名超重和74名肥胖男性。所有患者均在2013年1月至2015年1月期间被转诊至山西省妇幼保健院（中国太原）生殖医学中心。本研究的目的是探讨白藜芦醇治疗对肥胖男性精子活力、血浆锌浓度和顶体酶活性的影响。与体重正常组男性相比，超重和肥胖组男性的精子浓度、正常精子形态、精液量、DNA碎片率和睾酮水平显著降低。此外，与体重正常组相比，肥胖组的精子前向运动能力、精浆锌浓度和精子顶体酶活性显著降低。然而，与体重正常组相比，超重、肥胖和体重过轻组的雌二醇水平显著升高。值得注意的是，用0-100µmol/l白藜芦醇处理30分钟的弱精子症肥胖男性精液样本，精子活力有不同程度的改善。当这些精液样本用30µmol/l白藜芦醇处理时，与其他剂量的白藜芦醇相比，精子活力有所提高。因此，选择30µmol/l白藜芦醇进行进一步实验。用白藜芦醇（30µmol/l）处理精液样本30分钟后，实验组的精浆锌浓度和精子顶体酶活性与对照组相比显著增加。这些数据表明，男性肥胖不仅通过改变激素水平，还通过直接改变精子功能对男性生殖潜力产生负面影响。此外，白藜芦醇可能对肥胖引起的精液异常具有治疗和保护作用。
