Schmidt Elizabeth M S, Kjelgaard-Hansen Mads, Thomas Funmilola, Tvarijonaviciute Asta, Cerón José J, Eckersall P David
Department of Veterinary Clinical Sciences, School of Veterinary Medicine and Animal Science, São Paulo State University (FMVZ-UNESP), Botucatu, Brazil.
Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Vet Clin Pathol. 2016 Dec;45(4):657-664. doi: 10.1111/vcp.12400. Epub 2016 Oct 17.
Dioctophyme renale is a nematode parasite of dogs, usually found in the right kidney, causing severe damage to the renal parenchyma.
The objective was to evaluate the acute phase response in dogs naturally infected with this Giant Kidney Worm and the possible effects of nephrectomy on circulating concentrations of select acute phase proteins (APP) such as serum amyloid A (SAA), C-reactive protein (CRP), and haptoglobin (HP).
Nephrectomy was performed in infected dogs and the worms were collected for identification. Blood samples were taken 24 hours before surgery, and 4, 8, and 12 hours postoperatively on the following 10 consecutive days, and 28 days after surgery. Acute phase protein concentrations were determined at all time points. Cortisol concentrations were determined 24 hours before surgery and at recovery (28 days after surgery). One-way ANOVA and Friedman test were used for multiple comparisons; the Wilcoxon-signed rank test was used to compare variables, and Spearman's rho rank test was used to assess the correlation between the number of parasites recovered from the dogs and the APP concentration.
Forty-five parasites were recovered from the 12 dogs evaluated in this study. Dogs showed significantly increased HP concentrations (P < .05) but lower CRP and SAA concentrations before surgery, and cortisol concentrations were significantly higher at admission when compared to recovery. No significant correlations were found between the number of parasites and APP concentrations.
There is a particular acute phase response profile in dogs with kidney worm infection. Nephrectomy induced a short-term inflammatory process.
肾膨结线虫是犬类的一种线虫寄生虫，通常寄生于右肾，会对肾实质造成严重损害。
本研究旨在评估自然感染这种巨肾虫的犬的急性期反应，以及肾切除术对选定急性期蛋白（APP）循环浓度的可能影响，这些急性期蛋白包括血清淀粉样蛋白A（SAA）、C反应蛋白（CRP）和触珠蛋白（HP）。
对感染的犬进行肾切除术，并收集虫体进行鉴定。在手术前24小时、术后连续10天的术后4、8和12小时以及术后28天采集血样。测定所有时间点的急性期蛋白浓度。在手术前24小时和恢复时（术后28天）测定皮质醇浓度。采用单因素方差分析和Friedman检验进行多重比较；采用Wilcoxon符号秩检验比较变量，并采用Spearman秩相关检验评估从犬体内回收的寄生虫数量与APP浓度之间的相关性。
在本研究评估的12只犬中回收了45条寄生虫。犬在手术前HP浓度显著升高（P < 0.05），但CRP和SAA浓度较低，与恢复时相比，入院时皮质醇浓度显著更高。未发现寄生虫数量与APP浓度之间存在显著相关性。
感染肾虫的犬存在特定的急性期反应特征。肾切除术引发了短期炎症过程。
