Matringe M, Camadro J M, Labbe P, Scalla R
Laboratoire des Herbicides, INRA, BV 1540, Dijon, France.
Biochem J. 1989 May 15;260(1):231-5. doi: 10.1042/bj2600231.
Diphenyl ether herbicides induce an accumulation of protoporphyrin IX in plant tissues. By analogy to human porphyria, the accumulation could be attributed to decreased (Mg or Fe)-chelatase or protoporphyrinogen oxidase activities. Possible effects of acifluorfen-methyl on these enzymes were investigated in isolated corn (maize, Zea mays) etioplasts, potato (Solanum tuberosum) and mouse mitochondria, and yeast mitochondrial membranes. Acifluorfen-methyl was strongly inhibitory to protoporphyrinogen oxidase activities whatever their origins [concn. causing 50% inhibition (IC50) = 4 nM for the corn etioplast enzyme]. By contrast, it was roughly 100,000 times less active on (Mg or Fe)-chelatase activities (IC50 = 80-100 microM). Our results lead us to propose protoporphyrinogen oxidase as a cellular target for diphenyl ether herbicides.
二苯醚类除草剂会诱导植物组织中原卟啉IX的积累。类比人类卟啉症，这种积累可能归因于（镁或铁）螯合酶或原卟啉原氧化酶活性的降低。在分离的玉米（玉蜀黍，玉米）黄化质体、马铃薯（茄属）和小鼠线粒体以及酵母线粒体膜中研究了甲羧除草醚对这些酶的可能影响。无论原卟啉原氧化酶的来源如何，甲羧除草醚对其活性都有强烈抑制作用[对玉米黄化质体酶的半数抑制浓度（IC50）=4 nM]。相比之下，它对（镁或铁）螯合酶活性的活性约低100,000倍（IC50 = 80 - 100 microM）。我们的结果使我们提出原卟啉原氧化酶是二苯醚类除草剂的细胞靶点。
