Varano Mariaconcetta, Gaspari Marco, Quirino Angela, Cuda Giovanni, Liberto Maria Carla, Focà Alfredo
Institute of Microbiology, Department of Health Sciences, "Magna Graecia" University of Catanzaro, Catanzaro, Italy.
Laboratory Proteomics@UMG, Department of Experimental and Clinical Medicine, "Magna Graecia" University of Catanzaro, Catanzaro, Italy.
Proteomics. 2016 Dec;16(23):3019-3024. doi: 10.1002/pmic.201600048.
Ochrobactrum anthropi is a Gram-negative rod belonging to the Brucellaceae family, able to colonize a variety of environments, and actually reported as a human opportunistic pathogen. Despite its low virulence, the bacterium causes a growing number of hospital-acquired infections mainly, but not exclusively, in immunocompromised patients. The aim of this study was to obtain an overview of the global proteome changes occurring in O. anthropi in response to different growth temperatures, in order to achieve a major understanding of the mechanisms by which the bacterium adapts to different habitats and to identify some potential virulence factors. Combined quantitative mass spectrometry-based proteomics and bioinformatics approaches were carried out on two O. anthropi strains grown at temperatures miming soil/plants habitat (25°C) and human host environment (37°C), respectively. Proteomic analysis led to the identification of over 150 differentially expressed proteins in both strains, out of over 1200 total protein identifications. Among them, proteins responsible for heat shock response (DnaK, GrpE), motility (FliC, FlgG, FlgE), and putative virulence factors (TolB) were identified. The study represents the first quantitative proteomic analysis of O. anthropi performed by high-resolution quantitative mass spectrometry.
嗜人苍白杆菌是一种革兰氏阴性杆菌，属于布鲁氏菌科，能够在多种环境中定殖，实际上已被报道为人类机会致病菌。尽管其毒力较低，但该细菌主要在免疫功能低下的患者中引起越来越多的医院获得性感染，不过并非仅在这类患者中出现。本研究的目的是全面了解嗜人苍白杆菌在不同生长温度下发生的全球蛋白质组变化，以便更深入地了解该细菌适应不同栖息地的机制，并识别一些潜在的毒力因子。分别对在模拟土壤/植物栖息地温度（25°C）和人类宿主环境温度（37°C）下生长的两株嗜人苍白杆菌进行了基于定量质谱的蛋白质组学和生物信息学联合方法研究。蛋白质组学分析在总共鉴定出的1200多种蛋白质中，在两株菌中均鉴定出150多种差异表达蛋白质。其中，鉴定出了负责热休克反应的蛋白质（DnaK、GrpE）、运动性的蛋白质（FliC、FlgG、FlgE）以及假定的毒力因子（TolB）。该研究是首次通过高分辨率定量质谱对嗜人苍白杆菌进行的定量蛋白质组学分析。
