Watanabe Ryuichi, Sugai Chika, Yamazaki Taichi, Matsushima Ryoji, Uchida Hajime, Matsumiya Masahiro, Takatsu Akiko, Suzuki Toshiyuki
National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan.
Department of Marine Science and Resource, Nihon University, Kameino, Fujisawa 252-0880, Japan.
Toxins (Basel). 2016 Oct 13;8(10):294. doi: 10.3390/toxins8100294.
ERETIC2 (Electronic Reference To access In vivo Concentrations 2) based on PULCON (Pulse Length-based Concentration determination) methodology is a quantitative NMR (qNMR) using an external standard. The performance of the PULCON method was assessed using maleic acid (MA). Quantification of the diarrhetic shellfish toxin and okadaic acid by PULCON was successfully consistent with that obtained by a conventional internal standard method, demonstrating that the PULCON method is useful for the quantification of invaluable marine toxins without any contaminations by an internal standard.
基于脉冲长度浓度测定（PULCON）方法的电子参考体内浓度2（ERETIC2）是一种使用外标物的定量核磁共振（qNMR）方法。采用马来酸（MA）评估了PULCON方法的性能。通过PULCON对腹泻性贝类毒素和冈田酸进行定量，结果与传统内标法成功一致，表明PULCON方法可用于定量珍贵的海洋毒素，且不受内标物污染。
