Garg Monika, Tsujimoto Hisashi, Gupta Raj Kumar, Kumar Aman, Kaur Navneet, Kumar Rohit, Chunduri Venkatesh, Sharma Nand Kishor, Chawla Meenakshi, Sharma Saloni, Mundey Jaspreet Kaur
National Agri-Food Biotechnology Institute, Mohali-160071, Punjab, India.
United Graduate School of Agriculture, Tottori University, Tottori, Japan.
PLoS One. 2016 Oct 18;11(10):e0162350. doi: 10.1371/journal.pone.0162350. eCollection 2016.
Wheat cultivars with wide introgression have strongly impacted global wheat production. Aegilops geniculata (MgUg) is an important wild relative with several useful traits that can be exploited for wheat improvement. Screening of Ae. geniculata addition lines indicated a negative effect of 1Ug and the positive effect of 1Mg chromosome on wheat dough strength. Negative effect of 1Ug is probably associated with variation in number and position of the tripeptide repeat motif in the high molecular weight glutenin (HMW-G) gene. To utilize the positive potential of 1Mg chromosome, three disomic substitution lines (DSLs) 1Mg(1A), 1Mg(1B) and 1Mg(1D) were created. These lines were characterized for morphological, cytogenetic properties and biochemical signatures using FISH, 1D-, 2D-PAGE and RP-HPLC. Contribution of wheat 1A, 1B and 1D chromosomes towards dough mixing and baking parameters, chapatti quality, Fe/Zn content and glume color were identified. Observed order of variation in the dough mixing and baking parameters {1Mg(1D) ≤wheat ≤1Mg(1B) ≤1Mg(1A)} indicated that chromosome specific introgression is desirable for best utilization of wild species' potential.
具有广泛基因渗入的小麦品种对全球小麦生产产生了重大影响。节节麦(MgUg)是一种重要的野生近缘种,具有多个可用于改良小麦的有用性状。对节节麦附加系的筛选表明,1Ug染色体有负面影响,而1Mg染色体对小麦面团强度有正面影响。1Ug的负面影响可能与高分子量谷蛋白(HMW-G)基因中三肽重复基序的数量和位置变化有关。为了利用1Mg染色体的积极潜力,创建了三个二体代换系(DSLs)1Mg(1A)、1Mg(1B)和1Mg(1D)。使用荧光原位杂交(FISH)、一维聚丙烯酰胺凝胶电泳(1D-PAGE)、二维聚丙烯酰胺凝胶电泳(2D-PAGE)和反相高效液相色谱(RP-HPLC)对这些品系的形态学、细胞遗传学特性和生化特征进行了表征。确定了小麦1A、1B和1D染色体对面团揉混和烘焙参数、薄饼品质、铁/锌含量以及颖壳颜色的贡献。在面团揉混和烘焙参数方面观察到的变异顺序{1Mg(1D) ≤小麦 ≤1Mg(1B) ≤1Mg(1A)}表明,为了最佳利用野生种的潜力,特定染色体的基因渗入是可取的。