Wanderley Maria Carolina de Albuquerque, Neto José Manoel Wanderley Duarte, Filho José Luiz de Lima, Lima Carolina de Albuquerque, Teixeira José António Couto, Porto Ana Lúcia Figueiredo
Universidade Federal de Pernambuco (UFPE), Laboratório de Imunopatologia Keizo Asami (LIKA), Recife, PE, Brasil.
Universidade de Pernambuco (UPE), Faculdade de Ciências, Educação e Tecnologia de Garanhuns, Garanhuns, PE, Brasil.
Braz J Microbiol. 2017 Jan-Mar;48(1):13-24. doi: 10.1016/j.bjm.2016.08.001. Epub 2016 Sep 30.
Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenase) AND (fungi OR fungus OR fungal) AND (production OR synthesis OR synthesize) AND (characterization). Scientific criteria were adopted in this review to classify found articles by score (from 0 to 10). After exclusion criteria, 21 articles were selected. None obtained the maximum of 10 points defined by the methodology, which indicates a deficiency in studies dealing simultaneously with production, characterization and purification of collagenase by fungi. Among microorganisms studied the non-pathogenic fungi Penicillium aurantiogriseum and Rhizoctonia solani stood out in volumetric and specific collagenase activity. The only article found that made sequencing of a true collagenase showed 100% homology with several metalloproteinases fungi. A clear gap in literature about collagenase production by fungi was verified, which prevents further development in the area and increases the need for further studies, particularly full characterization of fungal collagenases with high specificity to collagen.
多年来一直需要能够降解天然三螺旋或变性胶原蛋白的特定蛋白酶，并且它们有广泛的应用。很少有完整的报告能全面揭示真菌胶原酶的生产、特性和纯化过程。在这篇综述中，作者使用以下关键词在四个科学在线数据库中进行搜索：（胶原分解的或胶原酶）且（真菌或菌类或真菌的）且（生产或合成或合成过程）且（特性）。本综述采用科学标准按分数（从0到10）对找到的文章进行分类。经过排除标准后，选择了21篇文章。没有一篇文章获得该方法定义的最高分10分，这表明在同时涉及真菌胶原酶的生产、特性和纯化的研究方面存在不足。在所研究的微生物中，非致病性真菌桔黄青霉和立枯丝核菌在体积和比胶原酶活性方面表现突出。唯一一篇对真正的胶原酶进行测序的文章显示，它与几种真菌金属蛋白酶有100%的同源性。已证实关于真菌产生胶原酶的文献存在明显空白，这阻碍了该领域的进一步发展，并增加了进一步研究的必要性，特别是对具有高胶原特异性的真菌胶原酶进行全面特性分析。
