Costa Luciana F, Mol Juliana P S, Silva Ana Patricia C, Macêdo Auricélio A, Silva Teane M A, Alves Geraldo E S, Winter Sebastian, Winter Maria G, Velazquez Eric M, Byndloss Mariana X, Bäumler Andreas J, Tsolis Renée M, Paixão Tatiane A, Santos Renato L
Departamento de Patologia, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária da Universidade Federal Minas Gerais, Belo Horizonte, MG, Brazil.
Int J Med Microbiol. 2016 Dec;306(8):604-610. doi: 10.1016/j.ijmm.2016.10.004. Epub 2016 Oct 13.
Salmonella enterica serotype Typhimurium is able to expand in the lumen of the inflamed intestine through mechanisms that have not been fully resolved. Here we utilized streptomycin-pretreated mice and dextran sodium sulfate (DSS)-treated mice to investigate how pathways for S. Typhimurium iron acquisition contribute to pathogen expansion in the inflamed intestine. Competitive infection with an iron uptake-proficient S. Typhimurium strain and mutant strains lacking tonB feoB, feoB, tonB or iroN in streptomycin pretreated mice demonstrated that ferric iron uptake requiring IroN and TonB conferred a fitness advantage during growth in the inflamed intestine. However, the fitness advantage conferred by ferrous iron uptake mechanisms was independent of inflammation and was only apparent in models where the normal microbiota composition had been disrupted by antibiotic treatment.
肠炎沙门氏菌鼠伤寒血清型能够通过尚未完全明确的机制在炎症肠道的肠腔中增殖。在此，我们利用链霉素预处理的小鼠和葡聚糖硫酸钠（DSS）处理的小鼠来研究鼠伤寒沙门氏菌获取铁的途径如何促进病原体在炎症肠道中的增殖。在链霉素预处理的小鼠中，用铁摄取能力正常的鼠伤寒沙门氏菌菌株与缺乏tonB、feoB、feoB、tonB或iroN的突变菌株进行竞争性感染，结果表明，需要IroN和TonB的三价铁摄取在炎症肠道生长过程中赋予了适应性优势。然而，二价铁摄取机制赋予的适应性优势与炎症无关，且仅在正常微生物群组成因抗生素治疗而被破坏的模型中才明显。
