Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84604, USA.
Viruses. 2024 Feb 13;16(2):289. doi: 10.3390/v16020289.
is a large family of Gram-negative bacteria composed of many pathogens, including and . Here, we characterize six bacteriophages that infect which were isolated from wastewater plants in the Wasatch front (Utah, United States). These phages are highly similar to the vB_SenM_Vi01 (Vi01), which was isolated using wastewater from Kiel, Germany. The phages vary little in genome size and are between 157 kb and 164 kb, which is consistent with the sizes of other phages in the Vi01-like phage family. These six phages were characterized through genomic and proteomic comparison, mass spectrometry, and both laboratory and clinical host range studies. While their proteomes are largely unstudied, mass spectrometry analysis confirmed the production of five hypothetical proteins, several of which unveiled a potential operon that suggests a ferritin-mediated entry system on the Vi01-like phage family tail. However, no dependence on this pathway was observed for the single host tested herein. While unable to infect every genus of tested, these phages are extraordinarily broad ranged, with several demonstrating the ability to infect and strains with generally high efficiency, as well as several clinical isolates, most likely due to their multiple tail fibers.
是一个包含许多病原体的革兰氏阴性菌大家族，其中包括 和 。在这里，我们描述了六种感染 的噬菌体，它们是从美国犹他州沃萨奇前缘的废水处理厂中分离出来的。这些噬菌体与在德国基尔使用废水分离出的 vB_SenM_Vi01（Vi01）高度相似。这些噬菌体的基因组大小差异不大，在 157kb 到 164kb 之间，这与 Vi01 样噬菌体家族中的其他噬菌体的大小一致。通过基因组和蛋白质组比较、质谱分析以及实验室和临床宿主范围研究对这六种噬菌体进行了表征。虽然它们的蛋白质组很大程度上尚未被研究，但质谱分析证实了五个假设蛋白的产生，其中一些揭示了一个潜在的操纵子，表明 Vi01 样噬菌体家族尾部存在铁蛋白介导的进入系统。然而，在此测试的单个宿主中没有观察到对该途径的依赖。虽然这些噬菌体不能感染测试的每个 属，但它们的宿主范围非常广泛，其中几种噬菌体能够以通常很高的效率感染 和 菌株，以及几种临床 分离株，这很可能是由于它们的多种尾部纤维。
