Department of Electronic Science, Kurukshetra University, Kurukshetra, 136119, India.
Department of Bio & NanoTechnology, Guru Jambheshwar University of Science and Technology, Hisar, 250001, India.
Environ Sci Pollut Res Int. 2017 Jan;24(1):926-937. doi: 10.1007/s11356-016-7774-y. Epub 2016 Oct 19.
Synthesis and controlled release study of polymeric nanoformulation of carbendazim (2-benzimidazole carbamic acid methyl ester) using chitosan and pectin is reported in this article. The formulation was subjected to morphological, physiological, in vitro fungicide release and bio-efficacy evaluation studies. The average size of nanoparticles was found to be in the range of 70-90 nm as confirmed by transmission electron microscopy. The in vitro fungicide release of nanoformulated carbendazim was compared with pure carbendazim at different pH values. The results confirmed sustained release of nanoformulated carbendazim. The bio-efficacy evaluation of the carbendazim nanoformulation was carried out against Fusarium oxysporum and Aspergillus parasiticus. The nanoformulation showed 100 % inhibition of test fungi at both concentrations (0.5 and 1.0 ppm) while pure carbendazim showed 80 ± 0 % and 97.2 ± 1.1 % inhibition at 0.5 and 1.0 ppm concentration respectively against Fusarium oxysporum and 86.0 ± 0.6 % inhibition and 100.0 % inhibition at 0.5 and 1.0 ppm concentration respectively against Aspergillus parasiticus. The commercial formulation (WP 50) showed 42 % and 58.0 ± 0.1 % inhibition at 0.5 and 1 ppm concentration respectively against Aspergillus parasiticus and 50.5 ± 0.7 % and 70.0 ± 0 % inhibition at 0.5 and 1.0 ppm concentrations respectively against Fusarium oxysporum. Phytotoxicity evaluation of nanoformulated fungicide confirmed that the nanoformulated carbendazim is safer for germination and root growth of the seeds of Cucumis sativa, Zea mays, and Lycopersicum esculantum.
本文报道了使用壳聚糖和果胶合成和控制释放多聚物纳米制剂的carbendazim（2-苯并咪唑氨基甲酸甲酯）的研究。该制剂进行了形态学、生理学、体外杀菌剂释放和生物功效评估研究。通过透射电子显微镜证实，纳米颗粒的平均粒径范围在 70-90nm 之间。在不同 pH 值下，比较了纳米制剂和纯 carbendazim 的体外杀菌剂释放。结果证实了纳米制剂 carbendazim 的持续释放。对 carbendazim 纳米制剂进行了对腐霉属和寄生曲霉的生物功效评估。纳米制剂在两种浓度（0.5 和 1.0ppm）下对测试真菌均表现出 100%的抑制作用，而纯 carbendazim 分别在 0.5 和 1.0ppm 浓度下对腐霉属的抑制率为 80±0%和 97.2±1.1%，在 0.5 和 1.0ppm 浓度下对寄生曲霉的抑制率分别为 86.0±0.6%和 100.0%。商业制剂（WP 50）分别在 0.5 和 1ppm 浓度下对寄生曲霉的抑制率为 42%和 58.0±0.1%，在 0.5 和 1.0ppm 浓度下对腐霉属的抑制率分别为 50.5±0.7%和 70.0±0%。纳米制剂杀菌剂的植物毒性评估证实，纳米制剂 carbendazim 对黄瓜、玉米和番茄种子的发芽和根系生长更安全。
