多聚免疫球蛋白受体的细胞内靶向信号在物种间高度保守。
Intracellular targetting signals of polymeric immunoglobulin receptors are highly conserved between species.
作者信息
Banting G, Brake B, Braghetta P, Luzio J P, Stanley K K
机构信息
European Molecular Biology Laboratory, Heidelberg, FRG.
出版信息
FEBS Lett. 1989 Aug 28;254(1-2):177-83. doi: 10.1016/0014-5793(89)81034-8.
A rat liver cDNA library, constructed in the plasmid expression vector pUEX, was immunoscreened using a rabbit polyclonal antiserum raised against rat liver Golgi membrane proteins. A sub-set of isolated clones were shown to encode the rat polymeric immunoglobulin receptor (pIgR). DNA sequence analysis of these clones provided the complete coding sequence of rat pIgR. Subsequent alignment of rat, rabbit and human predicted amino acid sequences demonstrated that the greatest degree of homology between the three pIgRs lies in their cytoplasmic tails; a region previously shown to be important for correct targetting and trancytosis of rabbit pIgR [(1984) Nature 308, 37-43].
利用针对大鼠肝高尔基体膜蛋白产生的兔多克隆抗血清，对构建于质粒表达载体pUEX中的大鼠肝cDNA文库进行免疫筛选。结果显示，分离出的一组克隆编码大鼠多聚免疫球蛋白受体（pIgR）。对这些克隆进行DNA序列分析，得到了大鼠pIgR的完整编码序列。随后对大鼠、兔和人预测的氨基酸序列进行比对，结果表明三种pIgR之间的最大同源性存在于它们的细胞质尾区；此前有研究表明，该区域对兔pIgR的正确靶向和转胞吞作用很重要[（1984年）《自然》308卷，37 - 43页]。
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