Luzio J P, Brake B, Banting G, Howell K E, Braghetta P, Stanley K K
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Biochem J. 1990 Aug 15;270(1):97-102. doi: 10.1042/bj2700097.
Organelle-specific integral membrane proteins were identified by a novel strategy which gives rise to monospecific antibodies to these proteins as well as to the cDNA clones encoding them. A cDNA expression library was screened with a polyclonal antiserum raised against Triton X-114-extracted organelle proteins and clones were then grouped using antibodies affinity-purified on individual fusion proteins. The identification, molecular cloning and sequencing are described of a type 1 membrane protein (TGN38) which is located specifically in the trans-Golgi network.
通过一种新策略鉴定了细胞器特异性整合膜蛋白，该策略可产生针对这些蛋白及其编码cDNA克隆的单特异性抗体。用针对经Triton X - 114提取的细胞器蛋白产生的多克隆抗血清筛选cDNA表达文库，然后使用在单个融合蛋白上亲和纯化的抗体对克隆进行分组。本文描述了一种特异性位于反式高尔基体网络的1型膜蛋白（TGN38）的鉴定、分子克隆和测序。
