Rajkumar Chellakannu, Thirumalraj Balamurugan, Chen Shen-Ming, Chen His-An
Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
J Colloid Interface Sci. 2017 Feb 1;487:149-155. doi: 10.1016/j.jcis.2016.10.024. Epub 2016 Oct 14.
In this study, we demonstrate a simple preparation of graphite (GR) sheets assisted with gelatin (GLN) polypeptide composite was developed for sensitive detection of dopamine (DA) sensor. The GR/GLN composite was prepared by GR powder in GLN solution (5mg/mL) via sonication process. The prepared GR/GLN composite displays well dispersion ability in biopolymer matrix and characterized via scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS) studies. The GR/GLN modified electrode showed an excellent electrocatalytic activity toward the oxidation of DA, suggesting that the successful formation of GR sheets crosslinked with the functional groups of GLN polypeptide. In addition, the GR/GLN modified electrode achieved a wide linear response ranging from 0.05 to 79.5μM with a detection limit of 0.0045μM. The calculated analytical sensitivity of the sensor was 1.36±0.02μAμMcm. Conversely, the modified electrode demonstrates a good storage stability, reproducibility and repeatability. In addition, the sensor manifests the determination of DA in human serum and urine samples for practical applications.
在本研究中，我们展示了一种借助明胶（GLN）多肽复合物的石墨（GR）片的简单制备方法，该方法用于灵敏检测多巴胺（DA）传感器。GR/GLN复合物通过将GR粉末置于GLN溶液（5mg/mL）中经超声处理制备而成。所制备的GR/GLN复合物在生物聚合物基质中显示出良好的分散能力，并通过扫描电子显微镜（SEM）、傅里叶变换红外（FTIR）光谱和电化学阻抗谱（EIS）研究进行了表征。GR/GLN修饰电极对DA的氧化表现出优异的电催化活性，这表明成功形成了与GLN多肽官能团交联的GR片。此外，GR/GLN修饰电极实现了0.05至79.5μM的宽线性响应，检测限为0.0045μM。传感器计算得出的分析灵敏度为1.36±0.02μAμMcm。相反，修饰电极表现出良好的储存稳定性、重现性和重复性。此外，该传感器还用于实际应用中检测人血清和尿液样本中的DA。
