Zhao Yong, Liu Xiao, Wang Xiaochen, Sun Chongyun, Wang Xinrui, Zhang Pingping, Qiu Jingfu, Yang Ruifu, Zhou Lei
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China; Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China.
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China; Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China; Chongqing Entry Exit Inspection and Quarantine Bureau, Chongqing 400020, PR China.
Talanta. 2016 Dec 1;161:297-303. doi: 10.1016/j.talanta.2016.08.058. Epub 2016 Aug 20.
Contamination of grains and other crops by aflatoxin B1 (AFB1), a highly toxic aflatoxin produced by Aspergillus flavus and Aspergillus parasiticus, poses a serious threat to human health and is an important food safety issue. In this study, a competitive up-converting phosphor technology-based lateral flow (AFB1-UPT-LF) assay was developed for rapid detection of AFB1. Detection sensitivity of the proposed assay can reach 0.03ngmL for standard AFB1 solutions, with the coefficients of variation (CV) less than 10% (from 1.0 to 9.4%). A good linearity (r=0.9889) was observed for quantification of AFB1 from 0.03 to 1000ngmL. Except for aflatoxin M1, no cross-reactivity was found with the abrin, ricin, ochratoxin A, botulinum toxin, shiga toxin 1, shiga toxin 2, and staphylococcal enterotoxin B, even at high concentrations of 100 or 1000ngmL. After optimizing the extraction of AFB1, the assay showed good tolerance to various crop samples, with the detection limit (from 0.1 to 5ngg) lower than the corresponding maximum residue level (MRL) set in China. The AFB1-UPT-LF assay provides a promising tool for rapid on-site detection of AFB1 because of its high sensitivity, specificity, and sample tolerance.
黄曲霉毒素B1（AFB1）是由黄曲霉和寄生曲霉产生的一种剧毒黄曲霉毒素，谷物和其他作物受其污染对人类健康构成严重威胁，是一个重要的食品安全问题。在本研究中，开发了一种基于竞争性上转换磷光技术的侧向流动（AFB1-UPT-LF）分析法用于快速检测AFB1。对于标准AFB1溶液，所提出的分析法的检测灵敏度可达0.03 ng/mL，变异系数（CV）小于10%（从1.0%到9.4%）。在0.03至1000 ng/mL范围内对AFB1进行定量时观察到良好的线性关系（r = 0.9889）。除黄曲霉毒素M1外，即使在100或1000 ng/mL的高浓度下，与相思子毒素、蓖麻毒素、赭曲霉毒素A、肉毒杆菌毒素、志贺毒素1、志贺毒素2和葡萄球菌肠毒素B均未发现交叉反应。在优化AFB1的提取后，该分析法对各种作物样品具有良好的耐受性，检测限（从0.1至5 ng/g）低于中国设定的相应最大残留限量（MRL）。AFB1-UPT-LF分析法因其高灵敏度、特异性和样品耐受性，为AFB1的快速现场检测提供了一种有前景的工具。
