Chang Ya-Chu, Lin Yu-Syuan, Xiao Guan-Ting, Chiu Tai-Chia, Hu Cho-Chun
Department of Applied Science, National Taitung University, Taiwan, ROC.
Department of Applied Science, National Taitung University, Taiwan, ROC; Agriculture Products Inspection Centre, National Taitung University, Taiwan, ROC.
Talanta. 2016 Dec 1;161:94-98. doi: 10.1016/j.talanta.2016.08.029. Epub 2016 Aug 10.
A turn-off fluorescence sensor synthesized by combining copper (II) oxide and multiwall carbon nanotubes (MWCNTs) were used for measuring glyphosate based on the inhibiting the catalytic activity of the CuO/MWCNTs. This sensor was synthesized by precipitating copper ions onto the acidic MWCNTs under basic conditions; the resulting material was characterized by the transmission electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy to confirm its structure. The CuO/MWCNTs nanomaterial was found to exhibit high peroxidase-like catalytic activity toward the reduction of HO to HO and the oxidation of Amplex Red to resorufin, with a corresponding color change from pink to red and the fluorescence enhancement. However, this activity was inhibited and the fluorescence diminished when glyphosate was added to the system. Using this strategy, we applied this sensor to detect glyphosate. The results indicated that this sensor is not only highly sensitive, with a detection limit of 0.67 ppb and a linear range from 0.002 to 0.01ppm, but also exhibits good selectivity for glyphosate. When this sensor was assessed for detecting glyphosate in real water samples, recoveries of 96-107% were attained. This proposed material and method are a promising approach for rapid screening of glyphosate.
通过将氧化铜（II）与多壁碳纳米管（MWCNTs）结合合成的一种关闭型荧光传感器，基于抑制CuO/MWCNTs的催化活性来用于测定草甘膦。该传感器是在碱性条件下将铜离子沉淀到酸性MWCNTs上合成的；所得材料通过透射电子显微镜、X射线光电子能谱和傅里叶变换红外光谱进行表征以确认其结构。发现CuO/MWCNTs纳米材料对将HO还原为HO以及将Amplex Red氧化为试卤灵表现出高的过氧化物酶样催化活性，伴随着相应的颜色从粉红色变为红色以及荧光增强。然而，当向系统中加入草甘膦时，这种活性受到抑制且荧光减弱。利用这种策略，我们将该传感器应用于检测草甘膦。结果表明该传感器不仅高度灵敏，检测限为0.67 ppb且线性范围为0.002至0.01 ppm，而且对草甘膦表现出良好的选择性。当评估该传感器用于检测实际水样中的草甘膦时，回收率达到了96 - 107%。这种提出的材料和方法是快速筛选草甘膦的一种有前景的方法。
