Coté Héloïse, Boucher Marie-Anne, Pichette André, Roger Benoit, Legault Jean
Laboratoire d'Analyse et de Séparation des Essences Végétales, Université du Québec à Chicoutimi, 555 boulevard de l'Université, Chicoutimi, QC, Canada G7G 4B7.
Laboratoire d'Analyse et de Séparation des Essences Végétales, Université du Québec à Chicoutimi, 555 boulevard de l'Université, Chicoutimi, QC, Canada G7G 4B7.
J Ethnopharmacol. 2016 Dec 24;194:684-689. doi: 10.1016/j.jep.2016.10.035. Epub 2016 Oct 18.
Oleoresin of Abies balsamea (L.) Mill. was used by Native Americans of the boreal forest of Canada and French Canadians to treat various infections, suggesting that oleoresin has antibacterial properties.
In this study, the antibacterial activity of whole oleoresin from A. balsamea was investigated against E. coli, S. aureus and two methicillin-resistant S. aureus (MRSA) strains using a new sensitive assay developed to evaluate hydrophobic matrix and compounds.
Antibacterial activity of oleoresin was first investigated using dilution and disk diffusion methods against E. coli and S. aureus, and compared to a new sensitive assay for hydrophobic matrix. Moreover, whole oleoresin was analyzed by GC-MS to characterize the composition and to identify the compounds responsible of the antibacterial activity.
The results showed that whole oleoresin was inactive against Gram-negative E. coli (MIC >90µg/ml) but active against Gram-positive S. aureus and MRSA with MIC ranging from 18.2 to 30µg/ml. The oleoresin is mainly composed of monoterpene (28%), sesquiterpenes (2%), and diterpenes (45%). Resin acids were found, in part, responsible for the antibacterial activity of whole oleoresin. Isopimaric acid and levopimaric acid are the most active with a MIC of respectively 9.7µg/ml and 10µg/ml.
This study supports the use of oleoresin of A. balsamea by the Native Americans and French Canadians to treat bacterial infections due to S. aureus.
加拿大北部森林的美洲原住民和法裔加拿大人曾使用香脂冷杉（Abies balsamea (L.) Mill.）的油树脂来治疗各种感染，这表明油树脂具有抗菌特性。
在本研究中，使用一种新开发的用于评估疏水基质和化合物的灵敏检测方法，研究了香脂冷杉全油树脂对大肠杆菌、金黄色葡萄球菌和两种耐甲氧西林金黄色葡萄球菌（MRSA）菌株的抗菌活性。
首先采用稀释法和纸片扩散法研究油树脂对大肠杆菌和金黄色葡萄球菌的抗菌活性，并与一种针对疏水基质的新灵敏检测方法进行比较。此外，通过气相色谱 - 质谱联用（GC - MS）分析全油树脂，以表征其成分并鉴定具有抗菌活性的化合物。
结果表明，全油树脂对革兰氏阴性菌大肠杆菌无活性（MIC>90μg/ml），但对革兰氏阳性菌金黄色葡萄球菌和MRSA有活性，MIC范围为18.2至30μg/ml。油树脂主要由单萜（28%）、倍半萜（2%）和二萜（45%）组成。发现树脂酸部分地导致了全油树脂的抗菌活性。异海松酸和左旋海松酸活性最强，MIC分别为9.7μg/ml和10μg/ml。
本研究支持美洲原住民和法裔加拿大人使用香脂冷杉油树脂治疗由金黄色葡萄球菌引起的细菌感染这一做法。
