Raie Diana S, Mhatre Eisha, Thiele Matthias, Labena A, El-Ghannam Gamal, Farahat Laila A, Youssef Tareq, Fritzsche Wolfgang, Kovács Ákos T
Process Design and Development Department, Egyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, Egypt.
Terrestrial Biofilms Group, Institute of Microbiology, Friedrich Schiller University Jena (FSU), Jena, Germany.
Mater Sci Eng C Mater Biol Appl. 2017 Jan 1;70(Pt 1):753-762. doi: 10.1016/j.msec.2016.09.038. Epub 2016 Sep 24.
The aim of this study was directed to reveal the repulsive effect of coated glass slides by quercetin and its bio-inspired titanium oxide and tungsten oxide nanoparticles on physical surface attachment of Bacillus subtilis as an ab-initio step of biofilm formation. Nanoparticles were successfully synthesized using sol-gel and acid precipitation methods for titanium oxide and tungsten oxide, respectively (in the absence or presence of quercetin). The anti-adhesive impact of the coated-slides was tested through the physical attachment of B. subtilis after 24h using Confocal Laser Scanning Microscopy (CLSM). Here, quercetin was presented as a bio-route for the synthesis of tungsten mixed oxides nano-plates at room temperature. In addition, quercetin had an impact on zeta potential and adsorption capacity of both bio-inspired amorphous titanium oxide and tungsten oxide nano-plates. Interestingly, our experiments indicated a contrary effect of quercetin as an anti-adhesive agent than previously reported. However, its bio-inspired metal oxide proved their repulsive efficiency. In addition, quercetin-mediated nano-tungsten and quercetin-mediated amorphous titanium showed anti-adhesive activity against B. subtilis biofilm.
本研究旨在揭示槲皮素及其仿生二氧化钛和氧化钨纳米颗粒对载玻片的修饰作用，以及这种修饰对枯草芽孢杆菌物理表面附着的排斥效应，这是生物膜形成的起始步骤。分别采用溶胶-凝胶法和酸沉淀法成功合成了氧化钛和氧化钨纳米颗粒（有无槲皮素存在）。通过共聚焦激光扫描显微镜（CLSM）检测修饰载玻片在24小时后对枯草芽孢杆菌物理附着的抗黏附影响。在此，槲皮素作为一种在室温下合成钨混合氧化物纳米片的生物途径被提出。此外，槲皮素对仿生非晶态二氧化钛和氧化钨纳米片的zeta电位和吸附容量有影响。有趣的是，我们的实验表明槲皮素作为抗黏附剂的作用与先前报道的相反。然而，其仿生金属氧化物证明了它们的排斥效率。此外，槲皮素介导的纳米钨和槲皮素介导的非晶态钛对枯草芽孢杆菌生物膜具有抗黏附活性。
