Biocorrosion Lab, Corrosion Protection Division, Council of Scientific & Industrial Research (CSIR) - Central Electrochemical Research Institute, Karaikudi 630 006, Tamilnadu, India.
J Photochem Photobiol B. 2012 May 2;110:43-9. doi: 10.1016/j.jphotobiol.2012.03.003. Epub 2012 Mar 17.
The nano-TiO(2) was synthesized biologically employing Bacillus subtilis (FJ460362). These nanoparticles were characterized by FTIR, TGA-DTA, UV-Visible spectroscopy, XRD and TEM. FTIR and TGA results confirm that the organic impurities were completely removed while calcinating the resultant products. Band gap value was estimated from the UV-Visible spectrum and anatase crystal phase was confirmed by XRD. TEM images reveal that these particles were agglomerated; mostly spherical in shape with an average particle size of 10-30nm. The synthesized nano-TiO(2) particles were coated on glass slides, biofilm were grown and subjected to irradiation of polychromatic light to understand photocatalytic activity in controlling the aquatic biofilm. The bacterial killing process was established by Epi-fluorescence microscopy. The results reveal that biogenic TiO(2) nanomaterial acts as good photocatalyst by the generation of H(2)O(2) in the vicinity of the TiO(2)-biofilm interfaces to suppress the growth of the aquatic biofilm.
采用枯草芽孢杆菌（FJ460362）生物合成了纳米 TiO(2)。这些纳米粒子通过傅里叶变换红外光谱（FTIR）、热重-差热分析（TGA-DTA）、紫外-可见光谱、X 射线衍射（XRD）和透射电子显微镜（TEM）进行了表征。FTIR 和 TGA 结果证实，在煅烧产物时，完全去除了有机杂质。从紫外-可见光谱估算了带隙值，并通过 XRD 证实了锐钛矿晶相。TEM 图像显示这些颗粒聚集在一起；主要呈球形，平均粒径为 10-30nm。将合成的纳米 TiO(2)颗粒涂覆在载玻片上，生长生物膜，并进行多色光照射，以了解控制水生生物膜的光催化活性。通过荧光显微镜观察细菌的杀灭过程。结果表明，生物生成的 TiO(2)纳米材料通过在 TiO(2)-生物膜界面附近生成 H(2)O(2)作为良好的光催化剂，抑制水生生物膜的生长。
