Brondz I, Olsen I
Research Department, National Institute of Occupational Health, Umeå, Sweden.
Infect Immun. 1989 Oct;57(10):3106-9. doi: 10.1128/iai.57.10.3106-3109.1989.
While Actinobacillus actinomycetemcomitans has been associated with rapidly progressive periodontal destruction in man, the closely related Haemophilus aphrophilus has not been related to periodontal disease. This may be due to differences in composition and structure of the lipopolysaccharides (LPS) of these dental-plaque bacteria, since LPS probably exerts a series of detrimental effects on the periodontium. LPS was prepared by the phenol-water procedure from the type strains of A. actinomycetemcomitans and H. aphrophilus, purified by hexane extraction and ultracentrifugation, and analyzed with gas chromatography and gas chromatography-mass spectrometry. While the lipid content of LPS from A. actinomycetemcomitans constituted 35.4%, it was only 18.4% in H. aphrophilus: 3-hydroxytetradecanoic and tetradecanoic acids were 21.1 and 14.3% in A. actinomycetemcomitans and 10.9 and 7.5% in H. aphrophilus. There were qualitative and quantitative differences in the polysaccharide portions of their LPS. A actinomycetemcomitans contained both D-glycero-D-mannoheptose and L-glycero-D-mannoheptose (7.8 and 11.3%); H. aphrophilus contained only L-glycero-D-mannoheptose (17.4%). The rhamnose, fucose, galactose, glucose, and glucosamine/galactosamine contents in A. actinomycetemcomitans were 2.6, 5.2, 10.1, 22.4, and 5.2%, respectively; in H. aphrophilus, they were 2.1, 2.6, 19.4, 36.4, and 3.7%. Chemical differences in LPS from A. actinomycetemcomitans and H. aphrophilus may contribute to the divergence in periodontopathogenic potential of these organisms and help taxonomic differentiation.
虽然伴放线放线杆菌与人的快速进展性牙周破坏有关，但密切相关的嗜沫嗜血杆菌与牙周疾病并无关联。这可能是由于这些牙菌斑细菌的脂多糖（LPS）在组成和结构上存在差异，因为LPS可能对牙周组织产生一系列有害影响。通过苯酚 - 水法从伴放线放线杆菌和嗜沫嗜血杆菌的模式菌株中制备LPS，经己烷萃取和超速离心纯化，并用气相色谱和气相色谱 - 质谱联用仪进行分析。伴放线放线杆菌LPS的脂质含量为35.4%，而嗜沫嗜血杆菌中仅为18.4%：3 - 羟基十四烷酸和十四烷酸在伴放线放线杆菌中分别为21.1%和14.3%，在嗜沫嗜血杆菌中分别为10.9%和7.5%。它们LPS的多糖部分存在质和量的差异。伴放线放线杆菌同时含有D - 甘油 - D - 甘露庚糖和L - 甘油 - D - 甘露庚糖（7.8%和11.3%）；嗜沫嗜血杆菌仅含有L - 甘油 - D - 甘露庚糖（17.4%）。伴放线放线杆菌中鼠李糖、岩藻糖、半乳糖、葡萄糖和氨基葡萄糖/半乳糖胺的含量分别为2.6%、5.2%、10.1%、22.4%和5.2%；在嗜沫嗜血杆菌中，它们分别为2.1%、2.6%、19.4%、36.4%和3.7%。伴放线放线杆菌和嗜沫嗜血杆菌LPS的化学差异可能导致这些微生物在牙周致病潜力上的差异，并有助于分类鉴别。
