Yoshida K, Yokozaki H, Niimoto M, Ito H, Ito M, Tahara E
First Department of Pathology, Hiroshima University School of Medicine, Japan.
Int J Cancer. 1989 Sep 15;44(3):394-8. doi: 10.1002/ijc.2910440303.
To elucidate the difference between scirrhous and non-scirrhous gastric carcinomas, we examined the expressions of TGF-beta, procollagen type I and type III in 7 gastric carcinoma cell lines and 37 gastric carcinoma tissues, and also examined the effect of TGF-beta on the expression of procollagen mRNA by TMK-I cells. TGF-beta mRNA was detected in all the tumors examined in vivo and in vitro. Interestingly, 9 (90%) of 10 scirrhous gastric carcinomas revealed higher levels of TGF-beta mRNA than normal tissues, while 8 (38%) of 21 well-differentiated adenocarcinomas had higher TGF-beta mRNA levels than normal tissues. As for procollagen mRNA, most of the human gastric carcinoma cell lines expressed type-I procollagen mRNA and MKN-I expressed type-III procollagen mRNA. Furthermore, procollagen type-I mRNA accumulation in TMK-I cells was increased by exogenous TGF-beta. Most of the tumor tissues from surgical specimens expressed higher procollagen mRNA than normal tissues. These results indicate that TGF-beta produced by carcinoma cells might stimulate collagen synthesis not only by fibroblasts but also by carcinoma cells themselves, leading to diffuse fibrosis of scirrhous gastric carcinomas.
为了阐明硬癌性和非硬癌性胃癌之间的差异，我们检测了7种胃癌细胞系和37例胃癌组织中转化生长因子-β（TGF-β）、I型和III型前胶原的表达情况，并研究了TGF-β对TMK-I细胞前胶原mRNA表达的影响。在体内和体外检测的所有肿瘤中均检测到TGF-β mRNA。有趣的是，10例硬癌性胃癌中有9例（90%）的TGF-β mRNA水平高于正常组织，而21例高分化腺癌中有8例（38%）的TGF-β mRNA水平高于正常组织。至于前胶原mRNA，大多数人胃癌细胞系表达I型前胶原mRNA，而MKN-I细胞系表达III型前胶原mRNA。此外，外源性TGF-β可增加TMK-I细胞中I型前胶原mRNA的积累。手术标本中的大多数肿瘤组织前胶原mRNA表达高于正常组织。这些结果表明，癌细胞产生的TGF-β可能不仅刺激成纤维细胞，还刺激癌细胞自身的胶原合成，导致硬癌性胃癌的弥漫性纤维化。
