Istel Fabian, Schwarzmüller Tobias, Tscherner Michael, Kuchler Karl
Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
Bio Protoc. 2015 Jul 20;5(14). doi: 10.21769/BioProtoc.1528.
Here, we report a method for the transformation by electroporation of the human fungal pathogen (). The protocol can be used for transformations in single well or in 96-well microtiter plates. It has been extensively used to generate a genome-scale gene deletion library using the background recipient strain ATCC2001 (Schwarzmüller , 2014).
在此,我们报告了一种通过电穿孔转化人类真菌病原体()的方法。该方案可用于单孔或96孔微量滴定板中的转化。它已被广泛用于使用背景受体菌株ATCC2001(施瓦茨米勒,2014年)构建全基因组规模的基因缺失文库。