D'Spain Samantha, Andrade Pilar I, Brockman Nohelli E, Fu Jianmin, Wickes Brian L
The Department of Microbiology, Immunology, and Molecular Genetics, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA.
J Fungi (Basel). 2022 Jun 2;8(6):596. doi: 10.3390/jof8060596.
The use of broad-spectrum antimycotic therapy, immunosuppressive therapy, and indwelling medical devices has contributed to the increased frequency of mucosal and systemic infections caused by . A major concern for and other spp. infections is the increase in drug resistance. To address these issues, additional molecular tools for the study of are needed. In this investigation, we developed an transformation system for . A number of parameters were investigated to determine their effect on transformation frequency, and then an optimized protocol was developed. The optimal conditions for the transformation of were found to be an infection incubation temperature of 26 °C, 0.2 mM acetosyringone in both induction media and co-culture media, 0.7% agar concentration, and a multiplicity of infection of 50:1 to . Importantly, the frequency of multiple integrations was low (5%), demonstrating that generally integrates at single sites in , which is consistent with other fungal transformation systems. The development of this system in adds another tool for the molecular manipulation of this increasingly important fungal pathogen.
广谱抗真菌治疗、免疫抑制治疗以及留置医疗设备的使用导致了由……引起的黏膜和全身感染频率增加。对于……和其他……属感染而言，一个主要担忧是耐药性的增加。为解决这些问题，需要更多用于研究……的分子工具。在本研究中，我们开发了一种用于……的转化系统。研究了多个参数以确定它们对转化频率的影响，随后制定了优化方案。发现用于……转化的最佳条件为感染培养温度26℃、诱导培养基和共培养基中均含有0.2 mM乙酰丁香酮、琼脂浓度0.7%以及感染复数为50:1……至……。重要的是，多重整合频率较低（5%），这表明……通常在……中以单一位点整合，这与其他真菌……转化系统一致。在……中开发此系统为对这种日益重要的真菌病原体进行分子操作增添了另一种工具。
