Wang Y-F, Zhang S, Li X-Q, Wang Y
Department of Gynaecology and Obstetrics, Henan Provincial People's Hospital and Zhengzhou University People's Hospital, Zhengzhou, China.
Eur Rev Med Pharmacol Sci. 2016 Oct;20(19):3987-3991.
To investigate Long non-coding RNA HULC (HULC) expression in cervical cancer and to evaluate its clinical significance.
HULC expression in 244 pairs of human cervical cancer and adjacent normal tissues was detected by real-time quantitative RT-PCR assay. The correlation between HULC and clinicopathological factors was also evaluated. The clinical and prognostic significance of HULC expression was analyzed statistically by Kaplan-Meier estimate and Cox regression model.
HULC expression was high in cervical cancer (p < 0.01). Also, the high HULC expression was significantly associated with the FIGO stage, lymph node metastasis and depth of cervical invasion (p < 0.05). Kaplan-Meier analysis showed that cervical cancer pa¬tients with high expression of HULC possessed poorer outcome than those with low expression of HULC (p < 0.001). Based on the univariate and multivariate analysis, the elevated expression of the HULC protein was a significant predictor of poor prognosis for cervical cancer patients (p = 0.001).
Our data firstly showed that the expression of HULC was upregulated in cervical cancer, and associated with overall survival, indicating that HULC may serve as a predictive biomarker for the prognosis of cervical cancer.
研究长链非编码RNA HULC（HULC）在宫颈癌中的表达情况，并评估其临床意义。
采用实时定量逆转录聚合酶链反应（RT-PCR）检测244对人宫颈癌组织及癌旁正常组织中HULC的表达。同时评估HULC与临床病理因素之间的相关性。采用Kaplan-Meier法和Cox回归模型对HULC表达的临床及预后意义进行统计学分析。
宫颈癌组织中HULC表达较高（p < 0.01）。此外，HULC高表达与国际妇产科联盟（FIGO）分期、淋巴结转移及宫颈浸润深度显著相关（p < 0.05）。Kaplan-Meier分析显示，HULC高表达的宫颈癌患者预后较HULC低表达者差（p < 0.001）。基于单因素和多因素分析，HULC蛋白表达升高是宫颈癌患者预后不良的重要预测指标（p = 0.001）。
我们的数据首次表明，HULC在宫颈癌中表达上调，并与总生存期相关，提示HULC可能作为宫颈癌预后的预测生物标志物。
