Institute for Biological and Medical Imaging, Helmholtz Zentrum München , 85764 Neuherberg, Germany.
Institute of Developmental Genetics, Helmholtz Zentrum München , 85764 Neuherberg, Germany.
Anal Chem. 2016 Nov 15;88(22):10785-10789. doi: 10.1021/acs.analchem.6b03039. Epub 2016 Oct 31.
Photoacoustic imaging (PAI) is an attractive imaging modality that can volumetrically map the distribution of photoabsorbing molecules with deeper tissue penetration than multiphoton microscopy. To enable dynamic sensing of divalent cations via PAI, we have engineered a new reversible near-infrared probe that is more sensitive to calcium as compared to other biologically relevant cations. The metallochromic compound showed a strong reduction of its peak absorbance at 765 nm upon addition of calcium ions that was translated into robust signal changes in photoacoustic images. Therefore, the heptamethine cyanine dye will be an attractive scaffold to create a series of metallochromic sensors for molecular PAI.
光声成像是一种很有吸引力的成像模式，与多光子显微镜相比，它可以对光吸收分子的分布进行体积成像，穿透更深的组织。为了通过光声成像实现对二价阳离子的动态感应，我们设计了一种新的近红外可逆探针，与其他生物相关的阳离子相比，该探针对钙的灵敏度更高。金属络合物在加入钙离子后，其在 765nm 处的峰值吸收明显降低，这转化为光声图像中强大的信号变化。因此，七甲川花菁染料将成为构建一系列用于分子光声成像的金属络合传感器的有吸引力的支架。
