Ma Jun, Wang Jun, Fan Weifei, Pu Xiaolin, Zhang Dawei, Fan Chou, Xiong Lin, Zhu Huijun, Xu Ning, Chen Renjie, Liu Shaofeng
Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College No. 92 Zheshan Xi Road, Wuhu 241000, China.
Department of Hematology and Oncology, Jiangsu Province Geriatric Institute No. 30 Luojia Road, Nanjing 210029, China.
Int J Clin Exp Pathol. 2013 Dec 15;7(1):246-54. eCollection 2014.
The tissue inhibitor of metalloproteinase-1 (TIMP-1) is an endogenous inhibitor of matrix metalloproteinases and potential biomarker of various types of human cancers. However, the association between TIMP-1 expression and the clinical features of laryngeal squamous cell carcinoma (LSCC) is barely investigated. In this study, one-step quantitative reverse transcription-polymerase chain reaction and immunohistochemical staining with tissue microarrays were employed to evaluate the relationship between TIMP-1 expression and the clinicopathological characteristics of LSCC. Results showed that the TIMP-1 mRNA and protein expression levels were significantly higher in LSCC than in the corresponding non-cancerous tissues (p<0.05). TIMP-1 protein expression in LSCC was associated with tumor differentiation (p=0.012) and overall survival (p=0.043). Kaplan-Meier method and Cox multi-factor analysis suggested that high TIMP-1 expression (p=0.008) and positive lymph node metastasis (p=0.029) were significantly associated with the poor survival of patients with LSCC. These data indicated that TIMP-1 may be identified as a prognostic marker of LSCC.
基质金属蛋白酶-1组织抑制剂（TIMP-1）是基质金属蛋白酶的内源性抑制剂，也是各类人类癌症的潜在生物标志物。然而，TIMP-1表达与喉鳞状细胞癌（LSCC）临床特征之间的关联鲜有研究。在本研究中，采用一步定量逆转录-聚合酶链反应以及组织芯片免疫组化染色来评估TIMP-1表达与LSCC临床病理特征之间的关系。结果显示，LSCC中TIMP-1 mRNA和蛋白表达水平显著高于相应的癌旁组织（p<0.05）。LSCC中TIMP-1蛋白表达与肿瘤分化（p=0.012）及总生存期（p=0.043）相关。Kaplan-Meier法和Cox多因素分析表明，TIMP-1高表达（p=0.008）及阳性淋巴结转移（p=0.029）与LSCC患者的不良生存显著相关。这些数据表明，TIMP-1可被鉴定为LSCC的预后标志物。
