Pedraza-Reyes M, Lopez-Romero E
Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Mexico.
J Gen Microbiol. 1989 Jan;135(1):211-8. doi: 10.1099/00221287-135-1-211.
Chitinase activity was measured in extracts of mycelial cells of Mucor rouxii as a function of the culture age. There was a peak of specific activity at the mid-exponential phase of growth (10 h), which paralleled chitin synthase activity. An additional peak of chitinase with higher specific activity was detected in 4 h cultures, which coincided with the onset of germination. Purification of chitinase activities from the cytoplasm revealed two enzymes, I and II, with different molecular mass and ionic charge. Antibodies induced with chitinase I did not cross-react with chitinase II. Both enzymes digested nascent chitin preferentially over preformed chitin, yielding diacetylchitobiose as the sole product of hydrolysis.
测定了鲁氏毛霉菌丝体细胞提取物中的几丁质酶活性与培养时间的关系。在生长的指数中期（10小时），比活性出现峰值，这与几丁质合酶活性平行。在4小时的培养物中检测到另一个比活性更高的几丁质酶峰值，这与萌发开始的时间一致。从细胞质中纯化几丁质酶活性，发现了两种酶，I和II，它们具有不同的分子量和离子电荷。用几丁质酶I诱导产生的抗体与几丁质酶II没有交叉反应。两种酶都优先消化新生几丁质而非预先形成的几丁质，水解的唯一产物是二乙酰壳二糖。
