水龙骨科复合体中微卫星标记的分离与表征
Isolation and characterization of microsatellite markers in the complex (Polypodiaceae).
作者信息
Zhao Cun-Feng, Kwak Myounghai, Xiang Qiao-Ping
机构信息
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Plant Resources Division, National Institute of Biological Resources, Incheon 404-708, Republic of Korea.
出版信息
Appl Plant Sci. 2016 Oct 12;4(10). doi: 10.3732/apps.1600069. eCollection 2016 Oct.
PREMISE OF THE STUDY
Microsatellites were designed and characterized in the Sino-Himalayan fern complex (Polypodiaceae) to further study the phylogeography and reproductive ecology of this species.
METHODS AND RESULTS
From a genomic library obtained by next-generation sequencing, 10 polymorphic and six monomorphic microsatellite loci were developed. In one population of from Taibaishan in central China, the number of alleles observed for these microsatellites ranged from seven to 29, and observed and expected heterozygosity ranged from 0.463 to 0.919 and from 0.797 to 0.947, respectively. Cross-amplification in other taxa within this complex was successful, but cross-amplification was poor for other congeneric species.
CONCLUSIONS
This set of newly developed microsatellite markers will be useful for assessing genetic diversity, population structure, and mating system, and to infer polyploid origin in the complex.
研究前提
设计并鉴定了中国喜马拉雅蕨类植物复合体（水龙骨科）中的微卫星，以进一步研究该物种的系统地理学和生殖生态学。
方法与结果
从通过下一代测序获得的基因组文库中开发了10个多态性微卫星位点和6个单态微卫星位点。在中国中部太白山的一个种群中，这些微卫星观察到的等位基因数从7到29不等，观察到的杂合度和预期杂合度分别为0.463至0.919和0.797至0.947。在该复合体内的其他分类群中交叉扩增成功，但在其他同属物种中交叉扩增效果不佳。
结论
这套新开发的微卫星标记将有助于评估该复合体中的遗传多样性、种群结构和交配系统，并推断多倍体起源。
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