非洲落叶乔木 Terminalia superba（使君子科）微卫星标记的开发与特征分析
Development and characterization of microsatellite markers in the African deciduous tree Terminalia superba (Combretaceae).
作者信息
Demenou Boris B, Migliore Jérémy, Tosso Felicien, Kaymak Esra, Hardy Olivier J
机构信息
Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium.
Management of Forest Resources, Biosystem Engineering Department, Gembloux Agro-Bio Tech, Université de Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium.
出版信息
Appl Plant Sci. 2015 Dec 9;3(12). doi: 10.3732/apps.1500070. eCollection 2015 Dec.
PREMISE OF THE STUDY
Microsatellites were designed and characterized in the African timber forest tree Terminalia superba (Combretaceae). Due to their high variability, these markers are suitable to investigate gene flow patterns and the structure of genetic diversity.
METHODS AND RESULTS
From a genomic library obtained by next-generation sequencing, seven monomorphic and 14 polymorphic microsatellite loci were developed. The polymorphic microsatellites displayed two to 27 alleles (mean 11.4; expected heterozygosity range 0.283-0.940, mean 0.736) in one population from southeastern Cameroon. Genotypes were typical of an outbreeding diploid species, although null alleles explain a significant heterozygote deficit in three loci. Cross-amplification in three congeneric species (T. ivorensis, T. avicennioides, and T. mantaly) failed, suggesting that T. superba is rather divergent.
CONCLUSIONS
This set of newly developed microsatellite markers will be useful for assessing the genetic diversity, population structure, and demographic history of T. superba in tropical African forests.
研究前提
在非洲用材林树种 Terminalia superba（使君子科）中设计并鉴定了微卫星。由于其高度变异性，这些标记适用于研究基因流动模式和遗传多样性结构。
方法与结果
从通过下一代测序获得的基因组文库中，开发了7个单态和14个多态微卫星位点。在喀麦隆东南部的一个种群中，多态微卫星显示出2至27个等位基因（平均11.4；预期杂合度范围0.283 - 0.940，平均0.736）。基因型是异交二倍体物种的典型特征，尽管无效等位基因解释了三个位点中显著的杂合子缺失。在三个同属物种（T. ivorensis、T. avicennioides 和 T. mantaly）中的交叉扩增失败，这表明 Terminalia superba 差异较大。
结论
这套新开发的微卫星标记将有助于评估热带非洲森林中 Terminalia superba 的遗传多样性、种群结构和种群历史。
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