Liu Rui, Wang Fei, Huang Qiong, Duan Jin-Ao, Liu Pei, Shang Erxin, Zhu Dong, Wen Hongmei, Qian Dawei
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing 210023, P.R. China.
Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, P.R. China.
Sci Rep. 2016 Oct 27;6:36027. doi: 10.1038/srep36027.
The use of endangered animal products in traditional Chinese medicine (TCM) and other ethno-medicines is culturally widespread across many regions of Asia. In the present study, traditional efficacies of seven types of animal horn including antipyretic, sedative and procoagulant activities were evaluated. Shotgun proteomic analysis was performed on material from horns following separation into soluble and insoluble fractions. Over 200 proteins were identified in each sample using nano LC-MS/MS, and these were classified according to their molecular function and cellular component using principal component analysis (PCA). The results indicated that seven horns showed antipyretic, sedative and procoagulant effect. Proteomic analysis showed that YH and WBH were similar to RH in terms of protein profile, and GH was similar to SAH. In addition, YH and GH were similar to RH in their cellular component classification profile. PCA based on the composition of keratin and keratin-associated proteins showed that constituents of WBH and GH were similar to RH and SAH, respectively. This is the first analysis of the protein content of animal horns used in TCM, and it is effective to substitute the horn of endangered animals with sustainable alternatives from domestic animals.
在传统中医（TCM）和其他民族医学中使用濒危动物制品在亚洲许多地区的文化中广泛存在。在本研究中，评估了七种动物角的传统功效，包括解热、镇静和促凝血活性。对角的材料进行分离，分为可溶部分和不溶部分后进行鸟枪法蛋白质组学分析。使用纳升液相色谱-串联质谱（nano LC-MS/MS）在每个样品中鉴定出200多种蛋白质，并使用主成分分析（PCA）根据它们的分子功能和细胞成分进行分类。结果表明，七种角显示出解热、镇静和促凝血作用。蛋白质组学分析表明，牦牛角（YH）和水牛角（WBH）在蛋白质谱方面与犀牛角（RH）相似，而山羊角（GH）与赛加羚羊角（SAH）相似。此外，牦牛角和山羊角在细胞成分分类谱方面与犀牛角相似。基于角蛋白和角蛋白相关蛋白组成的主成分分析表明，水牛角和山羊角的成分分别与犀牛角和赛加羚羊角相似。这是首次对传统中医中使用的动物角的蛋白质含量进行分析，用家畜的可持续替代品替代濒危动物的角是有效的。
