Institute of Physiology, The Czech Academy of Sciences, Prague, Czech Republic.
Kalmykian State University, Elista, Russia.
J Sep Sci. 2019 Feb;42(4):797-808. doi: 10.1002/jssc.201800910. Epub 2019 Jan 21.
Saiga horn extracts were analyzed with the goal of obtaining new information about compounds present in it. The purpose of this study is to find synthetic alternatives to Saiga horn extract, which is used in traditional Chinese medicine, by identifying potentially biologically active compounds in the extracts. Using high-performance liquid chromatography coupled with high-resolution mass spectrometry, we have been able to identify a series of short-chain polyhydroxybutyrates in alcoholic extracts of Saiga horn. Optimized high-performance liquid chromatography coupled with tandem mass spectrometry methods for analysis of short-chain poly-3-hydroxybutyrates were developed and subsequently applied to investigate Saiga horn extract for the presence of these compounds, which might explain its biological actions, particularly for its antipyretic and procoagulant properties.
对赛加羚羊角提取物进行分析，旨在获得有关其所含化合物的新信息。本研究的目的是通过鉴定提取物中具有潜在生物活性的化合物，找到替代赛加羚羊角提取物的合成物，赛加羚羊角提取物在中国传统医学中被使用。利用高效液相色谱-高分辨质谱联用技术，我们已经能够在赛加羚羊角的醇提物中鉴定出一系列短链聚羟基丁酸酯。优化了分析短链聚-3-羟基丁酸酯的高效液相色谱-串联质谱方法，并随后应用于研究赛加羚羊角提取物中这些化合物的存在情况，这些化合物可能解释其生物作用，特别是其解热和促凝血特性。
