Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), A.C., Unidad de Tecnología Alimentaria, Camino Arenero, 1227, El Bajío, Zapopan, Jalisco, México.
J Food Sci. 2019 May;84(5):1170-1179. doi: 10.1111/1750-3841.14503. Epub 2019 Apr 18.
High blood pressure can lead to cardiovascular diseases. The objective of this work was to obtain protein hydrolysates with antihypertensive potential from chia oil industry meal byproduct. Chia seed protein isolates (CPIs) were obtained from chia seed meal byproduct. CPI was hydrolyzed using different proteases (alcalase, pepsin, trypsin, and α-chymotrypsin) and their biological potential was evaluated using in vitro and in silico approaches. Chia seed pepsin protein hydrolysate showed the highest angiotensin-converting enzyme inhibition potential IC of 0.128 mg/mL (P < 0.05) compared to the rest of hydrolysates. Peptide sequence LIVSPLAGRL presented the lowest predicted binding energy and highest inhibition potential (-9.5 kcal/mol) compared to other sequenced peptides and positive controls (captopril and lisinopril). Chia peptides showed potential to block angiotensin-converting enzyme by interacting with its catalytic site. Chia seed oil industry meal byproduct could be used as an inexpensive source of protein and bioactive peptides with antihypertensive potential. PRACTICAL APPLICATION: This research shows an upcycling alternative for chia oil industry byproduct. Chia meal is a rich source of protein and can be used to generate bioactive peptides with antihypertensive potential. Chia protein isolate was obtained from chia meal and hydrolyzed using different enzymes, pepsin showed the highest antihypertensive potential. Chia meal waste could be a low-cost source of protein and protein hydrolysates that could be used as a food ingredient with antihypertensive potential.
高血压可导致心血管疾病。本工作的目的是从奇亚油工业副产物中获得具有降血压潜力的蛋白质水解物。从奇亚籽粕中获得奇亚籽蛋白分离物（CPI）。CPI 用不同的蛋白酶（碱性蛋白酶、胃蛋白酶、胰蛋白酶和α-糜蛋白酶）进行水解，并通过体外和计算机模拟方法评估其生物学潜力。与其他水解产物相比，奇亚籽胃蛋白酶水解产物的血管紧张素转换酶抑制潜力 IC 最高，为 0.128mg/mL（P<0.05）。与其他测序肽和阳性对照（卡托普利和赖诺普利）相比，肽序列 LIVSPLAGRL 表现出最低的预测结合能和最高的抑制潜力（-9.5kcal/mol）。奇亚肽通过与血管紧张素转换酶的催化位点相互作用显示出抑制血管紧张素转换酶的潜力。奇亚籽油工业副产物可用作具有降血压潜力的廉价蛋白质和生物活性肽的来源。实际应用：本研究为奇亚油工业副产物提供了一种升级利用的替代方案。奇亚籽粕是一种富含蛋白质的原料，可用于生成具有降血压潜力的生物活性肽。从奇亚籽粕中获得奇亚籽蛋白分离物，并使用不同的酶进行水解，胃蛋白酶显示出最高的降压潜力。奇亚籽粕废物可以作为具有降血压潜力的低成本蛋白质和蛋白质水解物的来源，可作为食品成分使用。
