Czelej Michał, Garbacz Katarzyna, Czernecki Tomasz, Wawrzykowski Jacek, Waśko Adam
Biolive Innovation Sp. z o. o., 3 Dobrzańskiego Street, 20-262 Lublin, Poland.
Department of Biotechnology, Microbiology and Human Nutrition, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, 8 Skromna Street, 20-704 Lublin, Poland.
Foods. 2022 Jun 30;11(13):1953. doi: 10.3390/foods11131953.
There is currently considerable interest on the use of animal, plant, and fungal sources in the production of bioactive peptides, as evidenced by the substantial body of research on the topic. Such sources provide cheap and environmentally friendly material as it often includes waste and by-products. Enzymatic hydrolysis is considered an efficient method of obtaining peptides capable of antioxidant activity. Those properties have been proven in terms of radical-scavenging capacity using the DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2-azinobis-(3-ethyl-benzothiazoline-6-sulphonic acid)), hydroxyl and superoxide radical methods. Additionally, the reducing power, ferrous ion-chelating (FIC), ferric reducing antioxidant power (FRAP), and the ability of the protein hydrolysates to inhibit lipid peroxidation have also been explored. The results collected in this review clearly indicate that the substrate properties, as well as the conditions under which the hydrolysis reaction is carried out, affect the final antioxidant potential of the obtained peptides. This is mainly due to the structural properties of the obtained compounds such as size or amino acid sequences.
目前，人们对利用动物、植物和真菌来源生产生物活性肽有着浓厚的兴趣，大量关于该主题的研究证明了这一点。这些来源提供了廉价且环保的材料，因为它们通常包括废物和副产品。酶水解被认为是获得具有抗氧化活性肽的有效方法。这些特性已通过使用DPPH（1,1-二苯基-2-苦基肼）和ABTS（2,2-联氮双（3-乙基苯并噻唑啉-6-磺酸））、羟基和超氧阴离子自由基方法的自由基清除能力得到证实。此外，还研究了蛋白质水解物的还原能力、亚铁离子螯合（FIC）、铁还原抗氧化能力（FRAP）以及抑制脂质过氧化的能力。本综述收集的结果清楚地表明，底物特性以及水解反应进行的条件会影响所得肽的最终抗氧化潜力。这主要是由于所得化合物的结构特性，如大小或氨基酸序列。
