交联官能度对纤维素纳米晶增强聚（乙烯醇）/海藻酸钠杂化支架的微观结构、力学性能和体外细胞相容性的影响。
Effect of crosslinking functionality on microstructure, mechanical properties, and in vitro cytocompatibility of cellulose nanocrystals reinforced poly (vinyl alcohol)/sodium alginate hybrid scaffolds.
机构信息
School of Chemical Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan 712 749, South Korea; Department of Nano, Medical and Polymer Materials, College of Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan 712 749, South Korea.
Department of Nano, Medical and Polymer Materials, College of Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan 712 749, South Korea.
出版信息
Int J Biol Macromol. 2017 Feb;95:962-973. doi: 10.1016/j.ijbiomac.2016.10.085. Epub 2016 Oct 25.
Cellulose nanocrystals reinforced poly (vinyl alcohol)/sodium alginate hybrid scaffolds were fabricated by using freeze casting and freeze drying method. In this study, the effect of crosslinking agents such as calcium chloride, orthophosphoric acid, and borax on morphological, structural, thermal, mechanical, and cytocompatibility (cell adhesion and proliferation) properties was investigated. The results showed that the change in type of crosslinking agent significantly changed the properties of the hybrid scaffolds. Based on this study, borax-crosslinked hybrid scaffold showed good fibrous porous structure with high porosity (95.2%), highest water uptake capacity, good thermal stability, mechanical stability (storage modulus), and in vitro cell adhesion and proliferation with fibroblast (NIH3T3) cells. This primarily research study explores the way for further use of this crosslinking agent to design and fabricate scaffolds for tissue engineering applications.
采用冷冻铸造和冷冻干燥法制备了纤维素纳米晶增强的聚乙烯醇/海藻酸钠杂化支架。本研究考察了交联剂氯化钙、正磷酸和硼砂对杂化支架的形态、结构、热性能、力学性能和细胞相容性（细胞黏附和增殖）的影响。结果表明，交联剂类型的变化显著改变了杂化支架的性能。基于这项研究，硼砂交联的杂化支架具有良好的纤维多孔结构，高孔隙率（95.2%）、最高的吸水率、良好的热稳定性、机械稳定性（储能模量）以及与成纤维细胞（NIH3T3 细胞）的体外黏附和增殖。这项初步研究探索了进一步使用这种交联剂来设计和制备组织工程应用支架的方法。
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