一种与伊朗蝎子毒液转录组中另外两种新型阳离子抗菌肽相关的新型防御素样肽。
A Novel Defensin-Like Peptide Associated with Two Other New Cationic Antimicrobial Peptides in Transcriptome of the Iranian Scorpion Venom.
作者信息
Baradaran Masoumeh, Jalali Amir, Naderi Soorki Maryam, Galehdari Hamid
机构信息
Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Pharmacology and Toxicology, Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Iran Biomed J. 2017 May;21(3):190-6. doi: 10.18869/acadpub.ibj.21.3.190. Epub 2017 Mar 1.
INTRODUCTION
Scorpion venom is a source of bioactive peptides, and some antimicrobial peptides (AMPs) have been found in the venom gland of scorpions. Therefore, the discovery of new anti-infective agents is an essential need to overcome the problem of antibiotic resistance of clinical isolates. Here, we describe three new cationic AMPs, including meuVAP-6, meuAP-18-1, and meuPep34 from the venom gland of the Iranian scorpion, Mesobuthus eupeus.
METHODS
The cDNA sequences encoding all the three peptides were obtained from the cDNA library of scorpion venom gland and were deposited in the GenBank database.
RESULTS
MeuVAP-6 and meuAP-18-1 are non-disulphide-bridged antimicrobial peptides, while meuPep34 is a cysteine-rich defensin-like peptide.
DISCUSSION
All three identified AMPs are rich in arginine and tryptophan. The overall results from the length, net charge, and hydrophobicity index suggested that meuPep34 could be the most active AMPs with the potential ability of biofilm inhibition. The data from molecular characterization of identified AMPs can provide a platform for further investigations in the drug design.
引言
蝎毒是生物活性肽的一个来源，并且在蝎子的毒腺中已发现一些抗菌肽（AMPs）。因此，发现新的抗感染剂对于克服临床分离株的抗生素耐药性问题至关重要。在此，我们描述了三种新的阳离子抗菌肽，包括来自伊朗蝎子中东金蝎（Mesobuthus eupeus）毒腺的meuVAP - 6、meuAP - 18 - 1和meuPep34。
方法
编码所有这三种肽的cDNA序列是从蝎毒腺的cDNA文库中获得的，并已存入GenBank数据库。
结果
MeuVAP - 6和meuAP - 18 - 1是无二硫键连接的抗菌肽，而meuPep34是一种富含半胱氨酸的防御素样肽。
讨论
所有三种鉴定出的抗菌肽都富含精氨酸和色氨酸。从长度、净电荷和疏水性指数得出的总体结果表明，meuPep34可能是最具活性的抗菌肽，具有抑制生物膜的潜在能力。已鉴定抗菌肽的分子特征数据可为药物设计的进一步研究提供一个平台。
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