用组织特异性生物分子治疗的雄性和雌性免疫缺陷大鼠的性别差异反应
Gender Difference Response of Male and Female Immunodeficiency Rats Treated with Tissue-specific Biomolecules.
作者信息
Fedulova Liliya V, Basov Alexandr A, Vasilevskaya Ekaterina R, Dzhimak Stepan S
机构信息
Experimental Clinic - Laboratory of Biologically Active Substances of Animal Origin, V.M. Gorbatov's Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow, Russian Federation.
Department of Fundamental and clinical biochemistry, Kuban State Medical University, Krasnodar, Russian Federation.
出版信息
Curr Pharm Biotechnol. 2019;20(3):245-253. doi: 10.2174/1389201020666190222184814.
BACKGROUND
The modern immunology is targeted to the detailed study of various immunopathological conditions at the molecular and cellular level, development of new methods for the prevention, diagnostics and treatment of contagious and non-contagious diseases of humans and animals.
METHODS
In the present work we took the rats with model of cyclophosphamide-induced immunodeficiency and studied the features of gender impact of the complex extract of immunocompetent organs (thymus, spleen and mesenteric lymph nodes) Sus scrofa and its separate fraction with molecular weight less than 30 kDa administered to male and female rats.
RESULTS
The impact of gender differences and tissue-specific biomolecules (30 kDa fraction) on hematological parameters (leukocytes, erythrocytes, platelets), functional activity of immune system (IL-2, IL-4, IL-6, complement system, IgG, IgM), biochemical parameters of hepatocytes functioning (activity of ALP and LDG), carbohydrate metabolism (glucose) and lipid metabolism (triglycerides).
CONCLUSION
Decrease of ALP activity is caused by inhibition of bile formation in a liver after introduction of cytostatic agent, and in contrast to complex extract, the administration of fraction 30 kDa allows improving bile production in male rats.
背景
现代免疫学旨在在分子和细胞水平上详细研究各种免疫病理状况，开发预防、诊断和治疗人类及动物传染性和非传染性疾病的新方法。
方法
在本研究中，我们采用环磷酰胺诱导免疫缺陷模型的大鼠，研究了将猪免疫活性器官（胸腺、脾脏和肠系膜淋巴结）的复合提取物及其分子量小于30 kDa的单独组分给予雄性和雌性大鼠后性别影响的特征。
结果
性别差异和组织特异性生物分子（30 kDa组分）对血液学参数（白细胞、红细胞、血小板）、免疫系统功能活性（IL-2、IL-4、IL-6、补体系统、IgG、IgM）、肝细胞功能生化参数（碱性磷酸酶和乳酸脱氢酶活性）、碳水化合物代谢（葡萄糖）和脂质代谢（甘油三酯）的影响。
结论
在引入细胞抑制剂后，碱性磷酸酶活性降低是由于肝脏胆汁形成受到抑制，与复合提取物相反，给予30 kDa组分可改善雄性大鼠的胆汁生成。
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