Bader C R, MacLeish P R, Schwartz E A
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3507-11. doi: 10.1073/pnas.75.7.3507.
Single, isolated rod photoreceptors were obtained by enzymatic dissociation of the tiger salamander (Ambystoma tigrinum) retina. These solitary cells retained the morphological features of rods of the intact retina and could be maintained in culture for several days. When impaled with micropipettes for electrophysiological recording, dark-adapted solitary rods had during darkness a resting potential of approximately -45 mV and a steady-state slope resistance of 500 Momega at rest. The current-voltage relationship showed both inward- and outward-going rectification. The responses to light of solitary rods were similar to those recorded from rods in the intact retina stimulated with large-diameter spots of light. The reversal potential of the light response of solitary rods was near 0 mV when measured in either the inner or outer segment.
通过酶解虎纹钝口螈（Ambystoma tigrinum）视网膜获得单个分离的视杆光感受器。这些单个细胞保留了完整视网膜视杆的形态特征，并且可以在培养中维持数天。当用微电极刺入进行电生理记录时，暗适应的单个视杆在黑暗中的静息电位约为-45 mV，静息时的稳态斜率电阻为500 MΩ。电流-电压关系显示出内向和外向整流。单个视杆对光的反应与完整视网膜中用大直径光斑刺激的视杆记录的反应相似。当在内段或外段测量时，单个视杆光反应的反转电位接近0 mV。
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