Muto Chiaki, Ishikawa Ryuji, Olsen Kenneth M, Kawano Kazuaki, Bounphanousay Chay, Matoh Toru, Sato Yo-Ichiro
Genetic Resources Center, National Agriculture and Food Research Organization , Tsukuba, Ibaraki 305-8602 , Japan.
Faculty of Agriculture and Life Science, Hirosaki University , Hirosaki, Aomori 036-8560 , Japan.
Breed Sci. 2016 Sep;66(4):580-590. doi: 10.1270/jsbbs.16032. Epub 2016 Jul 23.
A glutinous texture of endosperm is one of the important traits of rice ( L.). Northern Laos is known as a center of glutinous rice diversity. We genotyped INDEL, SSR and SNP markers in a sample of 297 rice landraces collected in northern Laos. These glutinous varieties were confirmed to share a loss-of-function mutation in (). INDEL markers revealed a high frequency of recombinant genotypes between and . Principal component analysis using SSR genotypes of flanking region revealed that glutinous landraces were scattered between non-glutinous and glutinous- types. High ratios of heterozygosity were found especially in glutinous . Haplotype analysis using SNP markers around locus revealed that glutinous landraces would have a few chromosome segments of glutinous . Frequent recombinations were confirmed outside of this region in glutinous . This intricate genetic structure of landraces suggested that glutinous landraces in Laos were generated through repeated natural crossing with glutinous- landraces and severe selection by local farmers.
胚乳的糯性质地是水稻的重要性状之一。老挝北部是公认的糯稻多样性中心。我们对在老挝北部收集的297份水稻地方品种样本中的插入缺失（INDEL）、简单序列重复（SSR）和单核苷酸多态性（SNP）标记进行了基因分型。这些糯性品种被证实共享了一个功能缺失突变。插入缺失标记显示在某基因与另一基因之间重组基因型的频率很高。利用某基因侧翼区域的SSR基因型进行主成分分析表明，糯性水稻地方品种散布于非糯性水稻和糯性某类型之间。尤其在糯性水稻中发现了高比例的杂合性。利用某基因座周围的SNP标记进行单倍型分析表明，糯性水稻地方品种会有一些糯性某染色体片段。在该区域之外的糯性水稻中证实了频繁的重组。地方品种这种复杂的遗传结构表明，老挝的糯性水稻地方品种是通过与糯性某地方品种反复自然杂交以及当地农民的严格选择而产生的。
