Cretoiu Dragos
Victor Babes National Institute of Pathology, Bucharest, 050096, Romania.
Division of Cellular and Molecular Biology and Histology, Carol Davila University of Medicine and Pharmacy, Bucharest, 050474, Romania.
Adv Exp Med Biol. 2016;913:325-334. doi: 10.1007/978-981-10-1061-3_21.
Lately, spatial three-dimensional (3D) identity of cells and their interrelations with the environment that surrounds it represent a challenging trend with the purpose to achieve a holistic view over the functions. Combining data from different imaging of cells in the third dimension can offer insight into behavior modalities making a world of difference. This chapter outlines a breakthrough in telocyte research by volume electron microscopy with the aid of focused ion beam scanning electron microscopy (FIB-SEM). Reconstructing 3D (three-dimensional) appearance of telocytes from a set of two-dimensional (2D) images by FIB-SEM tomography allowed to extract valuable data about their volume in nanoscale dimensions such as the three-dimensional morphology of telopodes and extracellular vesicles.
最近，细胞的空间三维（3D）特性及其与周围环境的相互关系代表了一种具有挑战性的趋势，旨在实现对功能的整体认识。将来自细胞在三维空间中不同成像的数据相结合，可以深入了解行为模式，这会带来巨大的差异。本章概述了借助聚焦离子束扫描电子显微镜（FIB-SEM）通过体积电子显微镜在端细胞研究方面取得的突破。通过FIB-SEM断层扫描从一组二维（2D）图像重建端细胞的三维（3D）外观，能够提取有关其纳米级尺寸体积的有价值数据，例如端粒的三维形态和细胞外囊泡。
