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通过基质辅助激光解吸电离飞行时间质谱对诺卡氏菌属菌种进行常规鉴定。

Routine identification of Nocardia species by MALDI-TOF mass spectrometry.

作者信息

Girard Victoria, Mailler Sandrine, Polsinelli Sophie, Jacob Delphine, Saccomani Marie Christine, Celliere Beatrice, Monnin Valerie, van Belkum Alex, Hagen Ferry, Meis Jacques F, Durand Géraldine

机构信息

R&D Microbiology, bioMérieux, La Balme Les Grottes, France.

R&D Microbiology, bioMérieux, La Balme Les Grottes, France.

出版信息

Diagn Microbiol Infect Dis. 2017 Jan;87(1):7-10. doi: 10.1016/j.diagmicrobio.2016.09.024. Epub 2016 Oct 4.

DOI:10.1016/j.diagmicrobio.2016.09.024
PMID:27802877
Abstract

We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0).

摘要

我们在此展示了通过VITEK质谱法对诺卡氏菌属细菌分离株进行充分的菌种鉴定。将特定的样品制备方法与强大的基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)数据库相结合,在一组164株分离株上实现了94%的菌种水平准确鉴定。使用MALDI-TOF MS鉴定诺卡氏菌属的功能将在VITEK MS更新的下一版本(IVD版本3.0)中提供。

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