快乐图谱：关于人类基因组连锁图谱绘制的一项提议。
Happy mapping: a proposal for linkage mapping the human genome.
作者信息
Dear P H, Cook P R
机构信息
Sir William Dunn School of Pathology, Oxford, UK.
出版信息
Nucleic Acids Res. 1989 Sep 12;17(17):6795-807. doi: 10.1093/nar/17.17.6795.
Abstract
A theoretical approach for linkage mapping the genome of any higher eukaryote is described. It uses the polymerase chain reaction, oligonucleotides of random sequence and single haploid cells. Markers are defined and then the DNA of a single sperm is broken at random (eg by gamma-rays) and physically split into 3 aliquots. Each aliquot is screened for the presence of each marker. Closely-linked markers are more likely to be found in the same aliquot than unlinked markers. The entire process is repeated with further sperm and the frequency that any two markers co-segregate determined. Closely-linked markers co-segregate from most cells; unlinked markers do so rarely. A map can then be constructed from these co-segregation frequencies. A specific application for determining the order and distance between sets of closely-linked and previously-defined markers is also described.
摘要
本文描述了一种用于对任何高等真核生物基因组进行连锁图谱分析的理论方法。该方法使用聚合酶链反应、随机序列的寡核苷酸和单倍体细胞。首先定义标记，然后将单个精子的DNA随机断裂（例如通过伽马射线）并物理分割成3份。对每份进行每个标记的存在情况筛选。紧密连锁的标记比不连锁的标记更有可能出现在同一份中。用更多精子重复整个过程，并确定任意两个标记共分离的频率。紧密连锁的标记在大多数细胞中共分离；不连锁的标记很少共分离。然后可以根据这些共分离频率构建图谱。还描述了该方法在确定紧密连锁且先前已定义的标记组之间的顺序和距离方面的具体应用。
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