基于质谱的蛋白质组学中的计算方法

Computational Methods in Mass Spectrometry-Based Proteomics.

作者信息

Li Sujun, Tang Haixu

机构信息

School of Informatics and Computing, Indiana University, Bloomington, IN, USA.

出版信息

Adv Exp Med Biol. 2016;939:63-89. doi: 10.1007/978-981-10-1503-8_4.

DOI:10.1007/978-981-10-1503-8_4

PMID:27807744

Abstract

This chapter introduces computational methods used in mass spectrometry-based proteomics, including those for addressing the critical problems such as peptide identification and protein inference, peptide and protein quantification, characterization of posttranslational modifications (PTMs), and data-independent acquisitions (DIA). The chapter concludes with emerging applications of proteomic techniques, such as metaproteomics, glycoproteomics, and proteogenomics.

摘要

本章介绍了基于质谱的蛋白质组学中使用的计算方法，包括用于解决诸如肽段鉴定和蛋白质推断、肽段和蛋白质定量、翻译后修饰（PTMs）表征以及数据非依赖采集（DIA）等关键问题的方法。本章最后介绍了蛋白质组学技术的新兴应用，如宏蛋白质组学、糖蛋白质组学和蛋白质基因组学。