Hsu Hsi-Hsien, Kuo Wei-Wen, Day Cecilia Hsuan, Shibu Marthandam Asokan, Li Shin-Yi, Chang Sheng-Huang, Shih Hui-Nung, Chen Ray-Jade, Viswanadha Vijaya Padma, Kuo Yueh-Hsiung, Huang Chih-Yang
Division of Colorectal Surgery, Mackay Memorial Hospital, Taipei, Taiwan.
Nursing Division, Mackay Medicine, Nursing and Management College, Taipei, Taiwan.
Environ Toxicol. 2017 Aug;32(8):2021-2031. doi: 10.1002/tox.22379. Epub 2016 Nov 3.
Taiwanin E is a natural compound which is structurally analogous to estrogen II and is abundantly found in Taiwania cryptomerioides. It has been previously reported for its anticancer effects; however, the pharmaceutical effect of Taiwanin E on Human LoVo colon cancer cells is not clear. In this study, we investigated the effects of Taiwanin E on metastasis and the associated mechanism of action on Human LoVo colon cancer cells with respect to the modulations in their cell migration and signaling pathways associated with migration. The results showed that Taiwanin E inhibited cell migration ability correlated with reduced expression and activity of MMP-2 and MMP-9. In addition, Taiwanin E induced activation of p38 through phosphorylation. Inhibition of p38α/β significantly abolished the effect of Taiwanin E on cell migration and MMP-2/-9 activity. Our results conclude that Taiwanin E inhibited cell migration chiefly via p38α MAPK pathway and in a lesser extend via p38β MAPK. The results elucidate the potential of the phytoestrogen natural compound Taiwanin E as a cancer therapeutic agent in inhibiting the cell migration. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 2021-2031, 2017.
台湾杉素E是一种天然化合物，其结构与雌激素II类似，在台湾杉中大量存在。此前已有关于其抗癌作用的报道；然而，台湾杉素E对人LoVo结肠癌细胞的药理作用尚不清楚。在本研究中，我们研究了台湾杉素E对人LoVo结肠癌细胞转移的影响及其相关作用机制，涉及细胞迁移和与迁移相关的信号通路的调节。结果表明，台湾杉素E抑制细胞迁移能力，这与MMP-2和MMP-9的表达及活性降低相关。此外，台湾杉素E通过磷酸化诱导p38激活。抑制p38α/β显著消除了台湾杉素E对细胞迁移和MMP-2/-9活性的影响。我们的结果表明，台湾杉素E主要通过p38α MAPK途径抑制细胞迁移，在较小程度上通过p38β MAPK途径。这些结果阐明了植物雌激素天然化合物台湾杉素E作为癌症治疗剂在抑制细胞迁移方面的潜力。© 2016威利期刊公司。《环境毒理学》32: 2021 - 2031, 2017。
