Xu Letian, Shi Zhanghong, Wang Bo, Lu Min, Sun Jianghua
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, China.
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China.
Int J Mol Sci. 2016 Nov 1;17(11):1734. doi: 10.3390/ijms17111734.
The exposure to plant defense chemicals has negative effects on insect feeding activity and modifies insect gut microbial community composition. is a very destructive forest pest in China, and harbors a large diversity and abundance of gut microorganisms. Host pine defensive chemicals can protect the pines from attack by the holobiont. In this study, boring length of feeding on 0 mg/g α-pinene and 9 mg/g α-pinene concentration in phloem media for 6 and 48 h were recorded, and their gut bacterial communities were analyzed in parallel. Nine milligram per gram α-pinene concentration significantly inhibited boring length of and altered its gut microbial community structure after 6 h. The inhibition of boring length from 9 mg/g α-pinene in diets ceased after 48 h. No significant differences of the bacterial communities were observed between the beetles in 0 and 9 mg/g α-pinene concentration in phloem media after 48 h. Our results showed that the inhibition of the feeding behavior of and the disturbance to its gut bacterial communities in 9 mg/g α-pinene concentration in phloem media after 6 h were eliminated after 48 h. The resilience of gut bacterial community of may help the beetle catabolize pine defense chemical.
接触植物防御化学物质会对昆虫的取食活动产生负面影响，并改变昆虫肠道微生物群落组成。在中国是一种极具破坏性的森林害虫，其肠道中栖息着种类繁多、数量丰富的微生物。寄主松树的防御化学物质可以保护松树免受这种共生体的攻击。在本研究中，记录了在韧皮部培养基中以0毫克/克和9毫克/克α-蒎烯浓度取食6小时和48小时的蛀道长度，并同时分析了它们的肠道细菌群落。9毫克/克α-蒎烯浓度在6小时后显著抑制了的蛀道长度，并改变了其肠道微生物群落结构。48小时后，9毫克/克α-蒎烯饮食对蛀道长度的抑制作用停止。48小时后，韧皮部培养基中0毫克/克和9毫克/克α-蒎烯浓度下的甲虫之间未观察到细菌群落的显著差异。我们的结果表明，48小时后，韧皮部培养基中9毫克/克α-蒎烯浓度下对取食行为的抑制及其肠道细菌群落的干扰被消除。肠道细菌群落的恢复力可能有助于该甲虫分解松树防御化学物质。
